HSP90 alpha Antibody (0R3S6)

Novus Biologicals | Catalog # NBP3-16528

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0R3S6 expressed in HEK293
View all HSP90 alpha Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HSP90 alpha (NP_005339.3). MPEETQTQDQPMEEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGKELHINLIPNKQDRTLTIVDTGIGMTK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP90 alpha Antibody (0R3S6)

Western Blot: HSP90 alpha Antibody (0R3S6) [NBP3-16528]

Western Blot: HSP90 alpha Antibody (0R3S6) [NBP3-16528]

Western Blot: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Western blot analysis of extracts of various cell lines, using HSP90 alpha Rabbit mAb (NBP3-16528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunoprecipitation: HSP90 alpha Antibody (0R3S6) [NBP3-16528]

Immunoprecipitation: HSP90 alpha Antibody (0R3S6) [NBP3-16528]

Immunoprecipitation: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug HSP90 alpha antibody (NBP3-16528). Western blot was performed from the immunoprecipitate using HSP90 alpha antibody (NBP3-16528) at a dilition of 1:1000.
HSP90 alpha Antibody (0R3S6)

Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] -

Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Confocal imaging of paraffin-embedded Rat brain tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
HSP90 alpha Antibody (0R3S6)

Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] -

Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Confocal imaging of paraffin-embedded Mouse brain tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
HSP90 alpha Antibody (0R3S6)

Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] -

Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Confocal imaging of paraffin-embedded Human colon cancer tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Applications for HSP90 alpha Antibody (0R3S6)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP90 alpha

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding,protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and playimportant roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress.There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutiveform. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM606219) (Chen et al., 2005 (PubMed 16269234)).(supplied by OMIM)

Long Name

Heat Shock Protein 90 alpha

Alternate Names

HSP86, HSP89A, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2

Gene Symbol

HSP90AA1

Additional HSP90 alpha Products

Product Documents for HSP90 alpha Antibody (0R3S6)

Certificate of Analysis

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Product Specific Notices for HSP90 alpha Antibody (0R3S6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail