HspB2 Antibody (4B9E2) - BSA Free

Novus Biologicals | Catalog # NBP2-52490

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4B9E2

Format

BSA Free
View all HspB2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human HSPB2 (AA: 1-182) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

20.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HspB2 Antibody (4B9E2) - BSA Free

Western Blot: HspB2 Antibody (4B9E2)BSA Free [NBP2-52490]

Western Blot: HspB2 Antibody (4B9E2)BSA Free [NBP2-52490]

Western Blot: HspB2 Antibody (4B9E2) [NBP2-52490] - Analysis using HSPB2 mAb against human HSPB2 (AA: 1-182) recombinant protein. (Expected MW is 46.2 kDa)
Flow Cytometry: HspB2 Antibody (4B9E2) - BSA Free [NBP2-52490]

Flow Cytometry: HspB2 Antibody (4B9E2) - BSA Free [NBP2-52490]

Flow Cytometry: HspB2 Antibody (4B9E2) [NBP2-52490] - Analysis of A549 cells using HSPB2 mouse mAb (green) and negative control (red).
Western Blot: HspB2 Antibody (4B9E2)BSA Free [NBP2-52490]

Western Blot: HspB2 Antibody (4B9E2)BSA Free [NBP2-52490]

Western Blot: HspB2 Antibody (4B9E2) [NBP2-52490] - Western blot analysis using HSPB2 mAb against HEK293 (1) and HSPB2 (AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: HspB2 Antibody (4B9E2) - BSA Free [NBP2-52490]

ELISA: HspB2 Antibody (4B9E2) - BSA Free [NBP2-52490]

ELISA: HspB2 Antibody (4B9E2) [NBP2-52490] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for HspB2 Antibody (4B9E2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HspB2

In muscle cells, HspB2 interacts with HspB3 to form a large independent polydisperse assembly. Unfortuantely, little is known about the function of this complex. However, co-immunoprecipitation has shown that despite a high level of sequence homology within the sHSP family, the HSPB2/B3 complex is distinctly different from the biochemical properties of other sHSPs. Therefore, the HSPB2/B3 assembly is believed to perform cellular functions that are distinctly different than those of its family members.

Alternate Names

DMPK-binding protein, HspB2, MKBP

Gene Symbol

HSPB2

Additional HspB2 Products

Product Documents for HspB2 Antibody (4B9E2) - BSA Free

Certificate of Analysis

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Product Specific Notices for HspB2 Antibody (4B9E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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