HTRA2/Omi Antibody (196C429)
Novus Biologicals | Catalog # NB100-56558
Key Product Details
Species Reactivity
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 196C429
Loading...
Product Specifications
Immunogen
This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 335-350 of human HtrA2.
Specificity
This antibody does not cross-react with mouse HtrA2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for HTRA2/Omi Antibody (196C429)
![Western Blot: HTRA2/Omi Antibody (196C429) [NB100-56558]](https://resources.rndsystems.com/images/products/HTRA2-Omi-Antibody-196C429-Western-Blot-NB100-56558-img0005.jpg "Western Blot: HTRA2/Omi Antibody (196C429) [NB100-56558]")
Western Blot: HTRA2/Omi Antibody (196C429) [NB100-56558]
Western Blot: HTRA2/Omi Antibody (196C429) [NB100-56558] - Detection of HtrA2 using an HtrA2 monoclonal antibody. HL-60 cell lysate was probed with HtrA2 antibody at 3 ug/ml and two protein bands of approximate molecular weight of 50 and 38 kDa were detected. The 50 kDa and 38 kDa bands may represent precursor and mature forms of HtrA2.![Immunohistochemistry: HTRA2/Omi Antibody (196C429) [NB100-56558]](https://resources.rndsystems.com/images/products/HTRA2-Omi-Antibody-196C429-Immunohistochemistry-NB100-56558-img0004.jpg "Immunohistochemistry: HTRA2/Omi Antibody (196C429) [NB100-56558]")
Immunohistochemistry: HTRA2/Omi Antibody (196C429) [NB100-56558]
Immunohistochemistry: HTRA2/Omi Antibody (196C429) [NB100-56558] - Analysis of HtrA2 using an HtrA2 monoclonal antibody. Probing with HtrA2 antibody shows staining in stomach tumor tissue (A) and very weak staining in normal stomach tissue (B).Applications for HTRA2/Omi Antibody (196C429)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
reported in scientific literature (Lee et al (2003))
Western Blot
2-3 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HTRA2/Omi
Long Name
High Temperature Requirement Protein-2
Alternate Names
Omi, PRSS25
Gene Symbol
HTRA2
Additional HTRA2/Omi Products
Product Documents for HTRA2/Omi Antibody (196C429)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HTRA2/Omi Antibody (196C429)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for HTRA2/Omi Antibody (196C429)
Powered by Bioz
Customer Reviews for HTRA2/Omi Antibody (196C429)
There are currently no reviews for this product. Be the first to review HTRA2/Omi Antibody (196C429) and earn rewards!
Have you used HTRA2/Omi Antibody (196C429)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
