Jurkat Whole Cell Lysate

Novus Biologicals | Catalog # NB800-PC2

Novus Biologicals
100% Guarantee

Key Product Details

Species

Human

Applications

SDS-PAGE, Western Blot

Product Specifications

Applications

Western Blot (reported in scientific literature)

Application Notes

This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.

Specificity

Jurkat whole cell lysate (Positive control)

Type

Cell

Tissue

Blood Lymph

Subcellular Fraction

Whole

Scientific Data Images for Jurkat Whole Cell Lysate

Western Blot: Jurkat Whole Cell Lysate [NB800-PC2]

Western Blot: Jurkat Whole Cell Lysate [NB800-PC2]

Western Blot: Jurkat Whole Cell Lysate [NB800-PC2] - Western blot analysis of Actin (NB600-532) using Jurkat whole cell lysate using NB800-PC2.
SDS-PAGE: Jurkat Whole Cell Lysate [NB800-PC2]

SDS-PAGE: Jurkat Whole Cell Lysate [NB800-PC2]

SDS-Page: Jurkat Whole Cell Lysate [NB800-PC2] - Ponceau S staining of Jurkat whole cell lysate using NB800-PC2.

Formulation, Preparation, and Storage

Formulation

HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Product Documents for Jurkat Whole Cell Lysate

Certificate of Analysis

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Product Specific Notices for Jurkat Whole Cell Lysate

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Citations for Jurkat Whole Cell Lysate

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