Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 499325
View all 5-HT1B Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human 5HT1B
Met1-Ser390
Accession # P28222

Specificity

Detects human 5HT1B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human 5-HT1B Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex and glia)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 5-HT1B

5HT1B is a 7TM receptor for serotonin and is expressed on striatal neurons and Purkinje cells in the cerebellum. 5HT1B ligation inhibits glycinergic signaling, appetite, circadian rhythm light responsiveness, and trigeminal-mediated nociception. It is an important pharmacologic target in the treatment of migrane headaches. Human 5HT1B shares 92% aa sequence identity with mouse and rat 5HT1B.

Long Name

5-Hydroxytryptamine Receptor 1B

Alternate Names

5HT1B, 5HT1DB, HTR1B, HTR1D2, HTR1DB, S12, Serotonin 1D beta receptor, Serotonin receptor 1B

Entrez Gene IDs

3351 (Human); 15551 (Mouse); 25075 (Rat)

Gene Symbol

HTR1B

UniProt

P28222

Additional 5-HT1B Products

Product Documents for Human 5-HT1B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human 5-HT1B Antibody

For research use only

Citations for Human 5-HT1B Antibody

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Protocols

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FAQs

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