Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG1 Clone # 2248B
View all ADAM28 Primary Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human ADAM28 protein
Leu72 & Leu191-Ala623, with a C-terminal 6-His tag
Accession # Q9UKQ2

Specificity

Detects human ADAM28 in direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG1

Applications for Human ADAM28 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human ADAM28 Monoclonal Antibody (Catalog # MAB9325).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAM28

A distintegrin and metalloprotease domain-containing protein 28 (ADAM28), also known as MDC-L, is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 80-90 kDa glycosylated proprotein that is processed to a mature form later in the secretory pathway (1-3). After the removal of the propeptide that contains a cysteine switch motif, the activated form of ADAM28 consists of a 467 aa extracellular domain (ECD) which contains a peptidase, disintegrin, cysteine-rich and EGF-like domains, followed by a 21 aa transmembrane segment and an 89 aa cytoplasmic domain (3). Alternative splicing generates additional isoforms with a variety of substitutions and deletions in the cysteine-rich and EGF-like domains (1). Within the ECD, human ADAM28 shares 73% aa sequence identity with mouse and rat ADAM28 (3).  ADAM28 is a cell surface protein that is involved in a variety of cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis (4-6), and exhibits catalytic activity to insulin-like growth factor binding protein-3 (7). Like many members of the ADAM proteins, the disintegrin domain of ADAM28 interacts with integrin to influence cell adhesion and cell-cell interaction (8). ADAM28 can mediate the adhesion of the Jurkat cells, T-lymphoma cell line, through integrin alpha 4b1(9).  R&D Systems in-house testing also indicates that ADAM28 cleaves IGFBP-3.

References

  1. Roberts, C.M. et al. (1999) J. Biol. Chem. 274:29251.
  2. McGinn, O.J. et al. (2011) Cell Biol. Int. 35:1043.
  3. Howard, L. et al. (2000) Biochem. J. 348:21.
  4. Mochizuki, S. and Okada Y, (2007) Cancer Sci. 98:621.
  5. Schlondorff, J. et al. (1999) J. Cell Sci. 112:3603.
  6. Blobel, C.P. (1997) Cell 90:589.
  7. Mochizuki S. and Okada Y. (2009) Curr. Pharm. Des. 15:2349.
  8. Edwards, L. et al. (2008) Molecular Aspects of Medicine 29:258.
  9. Bridges, L. et al. (2002) J. Biol. Chem. 277:3784.

Long Name

A Disintegrin and Metalloproteinase Domain 28

Alternate Names

Dtgn1, eMDCII, MDC-Lm, MDC-Ls, MDCL, Protein-L

Entrez Gene IDs

10863 (Human); 13522 (Mouse); 290344 (Rat)

Gene Symbol

ADAM28

UniProt

Q9UKQ2

Additional ADAM28 Products

Product Documents for Human ADAM28 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ADAM28 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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