Human ADAM28 Antibody

Recombinant Monoclonal Antibody
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Product Details
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Human ADAM28 Antibody Summary

Species Reactivity
Detects human ADAM28 in direct ELISA.
Recombinant Monoclonal Rabbit IgG1 Clone # 2248B
Protein A or G purified from cell culture supernatant
Chinese Hamster Ovary cell line, CHO-derived human ADAM28 protein
Leu72 & Leu191-Ala623, with a C-terminal 6-His tag
Accession # Q9UKQ2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human ADAM28 Monoclonal Antibody (Catalog # MAB9325).

This product is intended for assay development on various assay platforms requiring antibody pairs.


Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM28

A distintegrin and metalloprotease domain-containing protein 28 (ADAM28), also known as MDC-L, is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 80-90 kDa glycosylated proprotein that is processed to a mature form later in the secretory pathway (1-3). After the removal of the propeptide that contains a cysteine switch motif, the activated form of ADAM28 consists of a 467 aa extracellular domain (ECD) which contains a peptidase, disintegrin, cysteine-rich and EGF-like domains, followed by a 21 aa transmembrane segment and an 89 aa cytoplasmic domain (3). Alternative splicing generates additional isoforms with a variety of substitutions and deletions in the cysteine-rich and EGF-like domains (1). Within the ECD, human ADAM28 shares 73% aa sequence identity with mouse and rat ADAM28 (3).  ADAM28 is a cell surface protein that is involved in a variety of cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis (4-6), and exhibits catalytic activity to insulin-like growth factor binding protein-3 (7). Like many members of the ADAM proteins, the disintegrin domain of ADAM28 interacts with integrin to influence cell adhesion and cell-cell interaction (8). ADAM28 can mediate the adhesion of the Jurkat cells, T-lymphoma cell line, through integrin alpha 4b1(9).  R&D Systems in-house testing also indicates that ADAM28 cleaves IGFBP-3.

  1. Roberts, C.M. et al. (1999) J. Biol. Chem. 274:29251.
  2. McGinn, O.J. et al. (2011) Cell Biol. Int. 35:1043.
  3. Howard, L. et al. (2000) Biochem. J. 348:21.
  4. Mochizuki, S. and Okada Y, (2007) Cancer Sci. 98:621.
  5. Schlondorff, J. et al. (1999) J. Cell Sci. 112:3603.
  6. Blobel, C.P. (1997) Cell 90:589.
  7. Mochizuki S. and Okada Y. (2009) Curr. Pharm. Des. 15:2349.
  8. Edwards, L. et al. (2008) Molecular Aspects of Medicine 29:258.
  9. Bridges, L. et al. (2002) J. Biol. Chem. 277:3784.
Long Name
A Disintegrin and Metalloproteinase Domain 28
Entrez Gene IDs
10863 (Human); 13522 (Mouse); 290344 (Rat)
Alternate Names
a disintegrin and metalloproteinase domain 28; ADAM metallopeptidase domain 28; ADAM23MDC-L; ADAM28; disintegrin and metalloproteinase domain-containing protein 28; Dtgn1; EC 3.4.24; EC 3.4.24.-; eMDC II; eMDCII; Epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich proteinII; MDCL; MDCLADAM 28; MDC-Lm; MDC-Ls; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L; metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L; Protein-L

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