Human AGTR-2 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3659P

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Detection of AGTR-2 in NS0 Mouse Cell Line Transfected with AGTR-2 by Flow Cytometry.
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Human AGTR-2 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human AGTR‑2. Stains human AGTR-2-transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 364805
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human AGTR-2
Met1-Ser363
Accession # P50052
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of AGTR-2 antibody in NS0 Mouse Cell Line Transfected with AGTR-2 antibody by Flow Cytometry. View Larger

Detection of AGTR-2 in NS0 Mouse Cell Line Transfected with AGTR-2 by Flow Cytometry. NS0 mouse myeloma cell line transfected with AGTR-2 was stained with Mouse Anti-Human AGTR-2 PE-conjugated Monoclonal Antibody (Catalog # FAB3659P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: AGTR-2

AGTR-2, also known as AT2, is a 7-transmembrane protein that belongs to family 1 of G protein-coupled receptors. Both AGTR-2 and AGTR-1 bind Angiotensin II and function in the control of hemodynamics. In contrast to AGTR-1, which promotes vasoconstriction and cell proliferation, AGTR-2 inhibits growth and promotes apoptosis. In addition, Angiotensin II-induced AGTR-2 signaling induces the release of nitric oxide in the heart, kidney, and brain and participates in tissue morphogenesis and repair. Human AGTR-2 shares 92% amino acid sequence identity with mouse and rat AGTR-2.

Long Name
Angiostensin II Type 2 Receptor
Entrez Gene IDs
186 (Human); 11609 (Mouse); 24182 (Rat)
Alternate Names
AGTR2; AGTR-2; Angiostensin Receptor; angiotensin II receptor, type 2; Angiotensin II type-2 receptor; angiotensin receptor 2; AT2; AT2ATGR2; AT2R; MRX88; MRX88type-2 angiotensin II receptor

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