Human Aiolos/IKZF3 Alexa Fluor® 488-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC8625G-025
Detection of Aiolos/IKZF3 in Human PBMCs by Flow Cytometry.
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Human Aiolos/IKZF3 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Aiolos/IKZF3 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 914821
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human Aiolos/IKZF3
Met1-Gly54
Accession # Q9UKT9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Aiolos/IKZF3 in Human PBMCs by Flow Cytometry. View Larger

Detection of Aiolos/IKZF3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human Aiolos/IKZF3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8625G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Aiolos/IKZF3

IKAROS family zinc finger 3 (IKZF3), also known as Aiolos, is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development. This gene product interacts with IKZF family memebers as well as FoxP3, resulting in select gene silencing in Tregs. Over amino acids (aa) 1-54, human and mouse share 70% aa sequence identity.

Long Name
DNA-binding Protein Aiolos
Entrez Gene IDs
22806 (Human); 22780 (Mouse); 303511 (Rat)
Alternate Names
AIO; Aiolos; IKAROS family zinc finger 3 (Aiolos); Ikaros family zinc finger protein 3; IKZF3; zinc finger protein Aiolos; ZNFN1A3; ZNFN1A3subfamily 1A, 3 (Aiolos)

Product Datasheets

FAQs

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