Human Aiolos/IKZF3 Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody..
  • Species Reactivity
  • Specificity
    Detects human Aiolos/IKZF3 in direct ELISAs.
  • Source
    Recombinant Monoclonal Mouse IgG1 Clone # 914821
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    E. coli-derived recombinant human Aiolos/IKZF3
    Accession # Q9UKT9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Aiolos/IKZF3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human Aiolos/IKZF3 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC8625R) or (B) Mouse IgG1 Alexa Fluor 647 Isotype Control (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Aiolos/IKZF3
IKAROS family zinc finger 3 (IKZF3), also known as Aiolos, is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development. This gene product interacts with IKZF family memebers as well as FoxP3, resulting in select gene silencing in Tregs. Over amino acids (aa) 1-54, human and mouse share 70% aa sequence identity.
  • Long Name:
    DNA-binding Protein Aiolos
  • Entrez Gene IDs:
    22806 (Human); 22780 (Mouse); 303511 (Rat)
  • Alternate Names:
    AIO; AIOLOS; IKAROS family zinc finger 3 (Aiolos); Ikaros family zinc finger protein 3; IKZF3; zinc finger protein Aiolos; ZNFN1A3; ZNFN1A3subfamily 1A, 3 (Aiolos)
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC002R
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