Human CD19 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4867P
Human CD19 PE-conjugated Antibody in Data
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human CD19 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD19.
Source
Monoclonal Mouse IgG1 Clone # 4G7-2E3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human CLL cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD19 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD19 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD19

CD19 is a 95 kDa transmembrane glycoprotein with two Ig-like C2-set domains. CD19 regulates B cell development and activation through interactions with CD21, CD22, and the B cell receptor. CD19 polymorphisms and up‑regulation lead to the development of autoimmunity by promoting autoantibody production. Within the extracellular domain, human CD19 (Accession # P15391) shares 57% amino acid sequence identity with mouse and rat CD19.

Entrez Gene IDs
930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)
Alternate Names
B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12

Product Datasheets

Citations for Human CD19 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Effects of High Consumption of Vegetables on Clinical, Immunological, and Antioxidant Markers in Subjects at Risk of Cardiovascular Diseases
    Authors: I Peluso, A Raguzzini, G Catasta, V Cammisotto, A Perrone, C Tomino, E Toti, M Serafini
    Oxid Med Cell Longev, 2018;2018(0):5417165.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  2. Study of the microRNA expression profile of foreskin derived mesenchymal stromal cells following inflammation priming
    Authors: H Fayyad-Kaz, M Fayyad-Kaz, B Badran, D Bron, L Lagneaux, M Najar
    J Transl Med, 2017;15(1):10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Reviews for Human CD19 PE-conjugated Antibody

Average Rating: 5 (Based on 1 Review)

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Human CD19 PE-conjugated Antibody
By Anonymous on 04/10/2018
Application: Flow Sample Tested: B cell enriched peripheral blood mononuclear cells (PBMC) Species: Human