Human Angiopoietin-like Protein 2/ANGPTL2 Antibody

Catalog # Availability Size / Price Qty
AF2084
AF2084-SP
Detection of Human Angiopoietin‑like Protein 2/ANGPTL2 by Western Blot.
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Human Angiopoietin-like Protein 2/ANGPTL2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Angiopoietin-like 2 (ANGPTL2) in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse ANGPTL2 is observed and less than 1% cross-reactivity with recombinant human (rh) ANGPTL6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 2
Gln23-Phe492
Accession # Q9UKU9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Angiopoietin‑like Protein 2/ANGPTL2 by Western Blot. View Larger

Detection of Human Angiopoietin‑like Protein 2/ANGPTL2 by Western Blot. Western blot shows lysates of human heart tissue, human aorta tissue, and human uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Angiopoietin‑like Protein 2/ANGPTL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiopoietin‑like Protein 2/ANGPTL2 at approximately 55-64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 2/ANGPTL2

ANGPTL2, also known as angiopoietin-related protein 2 (ARP2), contains an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, which are structurally conserved in angiopoietins. Unlike angiopoietins which are ligands for TIE2, ANGPTL2 is an orphan ligand that does not bind TIE2.

Entrez Gene IDs
23452 (Human); 26360 (Mouse)
Alternate Names
angiopoietin-like 2; Angiopoietin-like Protein 2; angiopoietin-related protein 2; ANGPTL2; ANGRP2; ARP2HARP; MGC8889

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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