Human Angiopoietin-like Protein 2/ANGPTL2 Antibody

R&D Systems | Catalog # AF2084

R&D Systems
Discontinued Product
AF2084 has been discontinued. View all Angiopoietin-like Protein 2/ANGPTL2 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Angiopoietin-like Protein 2/ANGPTL2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 2
Gln23-Phe492
Accession # Q9UKU9

Specificity

Detects human Angiopoietin-like 2 (ANGPTL2) in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse ANGPTL2 is observed and less than 1% cross-reactivity with recombinant human (rh) ANGPTL6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Angiopoietin-like Protein 2/ANGPTL2 Antibody

Detection of Human Angiopoietin‑like Protein 2/ANGPTL2 antibody by Western Blot.

Detection of Human Angiopoietin‑like Protein 2/ANGPTL2 by Western Blot.

Western blot shows lysates of human heart tissue, human aorta tissue, and human uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Angiopoietin‑like Protein 2/ANGPTL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiopoietin‑like Protein 2/ANGPTL2 at approximately 55-64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Angiopoietin-like Protein 2/ANGPTL2 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human heart tissue, human aorta tissue, and human uterus tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 2/ANGPTL2

ANGPTL2, also known as angiopoietin-related protein 2 (ARP2), contains an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, which are structurally conserved in angiopoietins. Unlike angiopoietins which are ligands for TIE2, ANGPTL2 is an orphan ligand that does not bind TIE2.

Alternate Names

ANGPTL2, ANGRP2

Entrez Gene IDs

23452 (Human); 26360 (Mouse)

Gene Symbol

ANGPTL2

UniProt

Q9UKU9

Additional Angiopoietin-like Protein 2/ANGPTL2 Products

Product Documents for Human Angiopoietin-like Protein 2/ANGPTL2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Angiopoietin-like Protein 2/ANGPTL2 Antibody

For research use only

Citations for Human Angiopoietin-like Protein 2/ANGPTL2 Antibody

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FAQs

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Associated Pathways

Adipokines & Insulin Signaling Pathways
Adipokines & Insulin Signaling Pathway Thumbnail