Human APPL Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB7075S Datasheet / COA / SDS

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FAB7075S-100UG

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Human APPL Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human APPL in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG2b Clone # 709208

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human APPL
Met1-Ile373
Accession # Q9UKG1

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: APPL

APPL, also known as DCC interacting protein 13 alpha (DIP13 alpha), is a widely expressed approximately 80 kDa intracellular adaptor protein. APPL contains two coiled-coil domains (aa 215-259 and aa 621-673), a pleckstrin homology domain (aa 277-375) and a phosphotyrosine interaction domain (PID) (aa 496-656). These domains and the cytoplasmic and nuclear localization of APPL enable it to associate with a range of molecules involved in multiple pathways. APPL mediates the insulin-sensitizing and anti-inflammatory effects of Adiponectin through direct interactions with AdipoR1 and AdipoR2. Within aa 2-373, human APPL shares 99% aa sequence identity with mouse and rat APPL.

Long Name

Adapter Protein Containing PH Domain PTB Domain and Leucine Zipper Motif 1

Entrez Gene IDs

26060 (Human); 72993 (Mouse)

Alternate Names

Adapter protein containing PH domain, PTB domain and leucine zipper motif 1; adaptor protein containing pH domain, PTB domain and leucine zipper motif 1; adaptor protein, phosphotyrosine interaction, PH domain and leucine zippercontaining 1; AKT2 interactor; APPL; APPL1; APPLdip13-alpha; DCC-interacting protein 13-alpha; DIP 13 alpha; DIP13A; DIP13alpha; Dip13-alpha; KIAA1428; signaling adaptor protein DIP13alpha