Human ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # FAB4494AFP594

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 511702
View all ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Primary Antibodies »

Product Specifications

Specificity

Detects human ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse ASAHL is observed and no cross-reactivity with recombinant human ASAH2 or recombinant mouse ASAH2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase

The human NAAA gene encodes N-acylethanolamine-hydrolyzing acid amidase, also known as ASAH-like protein (ASAHL), a fatty acid amidase with maximal activity at acidic pH (1). ASAHL hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-, N-stearoyl, N-oleoyl, and N-arachidonoyl, but is most active against N‑palmitoylethanolamine (2). ASAHL is a member of the cholylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (3). ASAHL is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (3). ASAHL can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonyl fluorophosphonate (2).

References

  1. Hong, S.B. et al. (1999) Genomics 62:232.
  2. Ueda, N. et al. (2001) J. Biol. Chem. 276:35552.
  3. Tsuboi, K. et al. (2005) J. Biol. Chem. 280:11082.

Alternate Names

Acid ceramidase-like protein, ASAH-like protein, NAAA, Nacylethanolaminehydrolyzing Acid Amidase

Entrez Gene IDs

27163 (Human); 67111 (Mouse); 497009 (Rat)

Gene Symbol

NAAA

UniProt

Q02083

Additional ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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