Human Axl Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB154G
Human Axl Alexa Fluor® 488-conjugated Antibody in Data
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Product Details
Citations (3)
FAQs
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Human Axl Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Axl in direct ELISAs. In direct ELISAs, this antibody shows no cross-reactivity with recombinant mouse Axl, recombinant human (rh) Dtk or rhMer.
Source
Monoclonal Mouse IgG1 Clone # 108724
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Axl
Met1-Pro440
Accession # AAA61243
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below
Knockout Validated
Axl is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in Axl knockout A431 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Axl in HeLa Human Cell Line by Flow Cytometry. View Larger

Detection of Axl in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB154G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Axl in A431 Human Cell Line by Flow Cytometry. View Larger

Detection of Axl in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Alexa Fluor® 488-conjugated Mouse Anti-Human Axl Monoclonal Antibody (Catalog # FAB154G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. View Larger

Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. Axl knockout A431 human epithelial carcinoma cell line was stained with Alexa Fluor® 488-conjugated Mouse Anti-Human Axl Monoclonal Antibody (Catalog # FAB154G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). No staining in the Axl knockout A431 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1,2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References
  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.
Long Name
Axl Receptor Tyrosine Kinase
Entrez Gene IDs
558 (Human); 26362 (Mouse)
Alternate Names
AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL

Product Datasheets

Citations for Human Axl Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population
    Authors: R Leylek, M Alcántara-, Z Lanzar, A Lüdtke, OA Perez, B Reizis, J Idoyaga
    Cell Rep, 2019;29(11):3736-3750.e8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Sphingosine-1-Phosphate Receptor 1 Activity Promotes Tumor Growth by Amplifying VEGF-VEGFR2 Angiogenic Signaling
    Authors: VA Balaji Rag, M Anwar, MZ Akhter, A Chavez, Y Mao, V Natarajan, S Lakshmikan, M Chrzanowsk, AZ Dudek, L Claesson-W, JK Kitajewski, KK Wary, AB Malik, D Mehta
    Cell Rep, 2019;29(11):3472-3487.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. TAM receptor-dependent regulation of SOCS3 and MAPKs contributes to proinflammatory cytokine downregulation following chronic NOD2 stimulation of human macrophages.
    Authors: Zheng S, Hedl M, Abraham C
    J Immunol, 2015;194(4):1928-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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