Human Axl Antibody

Catalog # Availability Size / Price Qty
MAB154-SP
MAB154-100
MAB154-500
Human Axl Antibody in Data
3 Images
Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (2)

Human Axl Antibody Summary

Species Reactivity
Human
Specificity
Detects human Axl in direct ELISAs. In direct ELISAs, this antibody shows no cross-reactivity with recombinant mouse Axl, recombinant human (rh) Dtk or rhMer.
Source
Monoclonal Mouse IgG1 Clone # 108724
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Axl
Met1-Pro440
Accession # AAA61243
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Knockout Validated
Axl is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in Axl knockout A431 cell line.
 

Human Axl Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Axl Biotinylated Antibody (Catalog # BAF154)

Standard: Recombinant Human Axl Fc Chimera Protein, CF (Catalog # 154-AL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Axl in HeLa Human Cell Line by Flow Cytometry. View Larger

Detection of Axl in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Axl in A431 Human Cell Line by Flow Cytometry. View Larger

Detection of Axl in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. View Larger

Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. Axl knockout A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). No staining in the Axl knockout A431 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References
  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.
Long Name
Axl Receptor Tyrosine Kinase
Entrez Gene IDs
558 (Human); 26362 (Mouse)
Alternate Names
AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL

Product Datasheets

Citations for Human Axl Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Systemic Type I IFN Inflammation in Human ISG15 Deficiency Leads to Necrotizing Skin Lesions
    Authors: M Martin-Fer, M Bravo Garc, C Gruber, S Murias Loz, MNH Malik, F Alsohime, A Alakeel, R Valdez, S Buta, G Buda, MA Marti, M Larralde, B Boisson, M Feito Rodr, X Qiu, M Chrabieh, M Al Ayed, S Al Muhsen, JV Desai, EMN Ferre, SD Rosenzweig, B Amador-Bor, LY Bravo-Gall, R Olmer, S Merkert, M Bret, AK Sood, A Al-Rabiaah, MH Temsah, R Halwani, M Hernandez, F Pessler, JL Casanova, J Bustamante, MS Lionakis, D Bogunovic
    Cell Rep, 2020;31(6):107633.
    Species: Human
    Sample Types:
    Applications: CyTof
  2. Comprehensive Immunoprofiling of Pediatric Zika Reveals Key Role for Monocytes in the Acute Phase and No Effect of Prior Dengue Virus Infection
    Authors: D Michlmayr, EY Kim, AH Rahman, R Raghunatha, S Kim-Schulz, Y Che, S Kalayci, ZH Gümü?, G Kuan, A Balmaseda, A Kasarskis, SM Wolinsky, M Suaréz-Far, E Harris
    Cell Rep, 2020;31(4):107569.
    Species: Human
    Sample Types: Plasma
    Applications: CyTof
  3. MERTK acts as a costimulatory receptor on human CD8+ T cells
    Authors: MJW Peeters, D Dulkeviciu, A Draghi, C Ritter, A Rahbech, SK Skadborg, T Seremet, AM Carnaz Sim, E Martinenai, HR Halldórsdó, MH Andersen, G Holmen Olo, IM Svane, LJ Rasmussen, O Met, JC Becker, M Donia, C Desler, P Thor Strat
    Cancer Immunol Res, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Flow cytometry-based functional selection of RNA interference triggers for efficient epi-allelic analysis of therapeutic targets.
    Authors: Micklem D, Blo M, Bergstrom P, Hodneland E, Tiron C, Hoiby T, Gjerdrum C, Hammarsten O, Lorens J
    BMC Biotechnol, 2014;14(0):57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.
    Authors: Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K
    PLoS Pathog, 2014;10(12):e1004548.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry.
    Authors: Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y
    J. Virol., 2012;86(4):2067-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
    J. Exp. Med., 2012;209(11):2033-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
    Authors: Zhu D, Wang Y, Singh I
    Blood, 2010;115(23):4963-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Survival and migration of human dendritic cells are regulated by an IFN-alpha-inducible Axl/Gas6 pathway.
    Authors: Scutera S, Fraone T, Musso T, Cappello P, Rossi S, Pierobon D, Orinska Z, Paus R, Bulfone-Paus S, Giovarelli M
    J. Immunol., 2009;183(5):3004-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection
  10. Up-regulation of soluble Axl and Mer receptor tyrosine kinases negatively correlates with Gas6 in established multiple sclerosis lesions.
    Authors: Weinger JG, Omari KM, Marsden K, Raine CS, Shafit-Zagardo B
    Am. J. Pathol., 2009;175(1):283-93.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Multiple roles for the receptor tyrosine kinase axl in tumor formation.
    Authors: Holland SJ, Powell MJ, Franci C, Chan EW, Friera AM, Atchison RE, McLaughlin J, Swift SE, Pali ES, Yam G, Wong S, Lasaga J, Shen MR, Yu S, Xu W, Hitoshi Y, Bogenberger J, Nör JE, Payan DG, Lorens JB
    Cancer Res., 2005;65(20):9294-303.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Circulating levels of soluble MER in lupus reflect M2c activation of monocytes/macrophages, autoantibody specificities and disease activity.
    Authors: Zizzo G, Guerrieri J, Dittman L, Merrill J, Cohen P
    Arthritis Res Ther, 0;15(6):R212.
    Species: Human
    Sample Types: Plasma

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Reviews for Human Axl Antibody

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Human Axl Antibody
By Anonymous on 07/31/2020
Application: ELISA Sample Tested: Serum Species: Human

Human Axl Antibody
By Anonymous on 06/07/2016
Application: Func Sample Tested: NCI-H1299 Species: Human