Human Axl APC-conjugated Antibody
Human Axl APC-conjugated Antibody Summary
Met1-Pro440
Accession # AAA61243
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Axl in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Axl APC-conjugated Monoclonal Antibody (Catalog # FAB154A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of Axl in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with APC-conjugated Mouse Anti-Human Axl Monoclonal Antibody (Catalog # FAB154A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. Axl knockout A431 human epithelial carcinoma cell line was stained with APC-conjugated Mouse Anti-Human Axl Monoclonal Antibody (Catalog # FAB154A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). No staining in the Axl knockout A431 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Axl
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1,2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.
- Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Holland, S. et al. (2005) Canc. Res. 65:9294.
Product Datasheets
Citations for Human Axl APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein
Authors: H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim
Nature Medicine, 2022;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
AXL-Receptor Targeted 14FN3 Based Single Domain Proteins (Pronectins�) from 3 Synthetic Human Libraries as Components for Exploring Novel Bispecific Constructs against Solid Tumors
Authors: CA Hokanson, E Zacco, G Cappuccill, T Odineca, R Crea
Biomedicines, 2022;10(12):.
Species: Mouse
Sample Types: Whole Cell
Applications: Flow Cytometry -
The Most Common VHL Point Mutation R167Q in Hereditary VHL Disease Interferes with Cell Plasticity Regulation
Authors: S Buart, S Terry, MK Diop, P Dessen, S Couvé, A Abdou, J Adam, J Thiery, P Savagner, S Chouaib
Cancers, 2021;13(15):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Characterization of the Filovirus-Resistant Cell Line SH-SY5Y Reveals Redundant Role of Cell Surface Entry Factors
Authors: FJ Zapatero-B, E Dietzel, O Dolnik, K Döhner, R Costa, B Hertel, B Veselkova, J Kirui, A Klintworth, MP Manns, S Pöhlmann, T Pietschman, T Krey, S Ciesek, G Gerold, B Sodeik, S Becker, T von Hahn
Viruses, 2019;11(3):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Lipopolysaccharide inhalation recruits monocytes and dendritic cell subsets to the alveolar airspace
Authors: L Jardine, S Wiscombe, G Reynolds, D McDonald, A Fuller, K Green, A Filby, I Forrest, MH Ruchaud-Sp, J Scott, M Collin, M Haniffa, AJ Simpson
Nat Commun, 2019;10(1):1999.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Normal breast-derived epithelial cells with luminal and intrinsic subtype-enriched gene expression document inter-individual differences in their differentiation cascade
Authors: B Kumar, MS Prasad, P Bhat-Naksh, M Anjanappa, M Kalra, N Marino, AMV Storniolo, X Rao, S Liu, J Wan, Y Liu, H Nakshatri
Cancer Res., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Reviews for Human Axl APC-conjugated Antibody
Average Rating: 4.3 (Based on 3 Reviews)
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the gating was based on FMO control. In my hand, Axl antibody with APC works better than PE.
