Human Axl APC-conjugated Antibody

(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Axl in direct ELISAs. In direct ELISAs, this antibody shows no cross-reactivity with recombinant mouse Axl, recombinant human (rh) Dtk or rhMer.
  • Source
    Monoclonal Mouse IgG1 Clone # 108724
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Axl
    Met1-Pro440
    Accession # AAA61243
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
  • Knockout Validated
    Axl is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in Axl knockout A431 cell line.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Axl in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Axl APC‑conjugated Monoclonal Antibody (Catalog # FAB154A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Axl in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with APC-conjugated Mouse Anti-Human Axl Monoclonal Antibody (Catalog # FAB154A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Knockout Validated
Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. Axl knockout A431 human epithelial carcinoma cell line was stained with APC-conjugated Mouse Anti-Human Axl Monoclonal Antibody (Catalog # FAB154A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). No staining in the Axl knockout A431 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1,2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

  • References:
    1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
    2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
    3. Holland, S. et al. (2005) Canc. Res. 65:9294.
  • Long Name:
    Axl Receptor Tyrosine Kinase
  • Entrez Gene IDs:
    558 (Human); 26362 (Mouse)
  • Alternate Names:
    Ark; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; tyrosine-protein kinase receptor UFO; Ufo; UFOoncogene AXL
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 8  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human Axl APC-conjugated Antibody FAB154A
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Excellent
 Flow Human Anonymous 09/10/2016
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Flow Cytometry Human Axl APC-conjugated Antibody FAB154A
Flow Cytometry: Human Axl APC-conjugated Antibody [FAB154A]

Summary

ApplicationFlow Cytometry
Sample TestedASTROCYTE
SpeciesHuman
Flow Cytometry Human Axl APC-conjugated Antibody FAB154A
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Average
 Flow Human Anonymous 07/13/2016
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Image Details
Flow Cytometry Human Axl APC-conjugated Antibody FAB154A
Flow Cytometry: Human Axl APC-conjugated Antibody [FAB154A]

Summary

ApplicationFlow Cytometry
Sample TestedHuman AML cells
SpeciesHuman

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