Human Axl APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB154A
Human Axl APC-conjugated Antibody in Data
3 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (3)

Human Axl APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Axl in direct ELISAs. In direct ELISAs, this antibody shows no cross-reactivity with recombinant mouse Axl, recombinant human (rh) Dtk or rhMer.
Source
Monoclonal Mouse IgG1 Clone # 108724
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Axl
Met1-Pro440
Accession # AAA61243
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below
Knockout Validated
Axl is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in Axl knockout A431 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Axl in HeLa Human Cell Line by Flow Cytometry. View Larger

Detection of Axl in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Axl APC-conjugated Monoclonal Antibody (Catalog # FAB154A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Axl in A431 Human Cell Line by Flow Cytometry. View Larger

Detection of Axl in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with APC-conjugated Mouse Anti-Human Axl Monoclonal Antibody (Catalog # FAB154A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. View Larger

Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. Axl knockout A431 human epithelial carcinoma cell line was stained with APC-conjugated Mouse Anti-Human Axl Monoclonal Antibody (Catalog # FAB154A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). No staining in the Axl knockout A431 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1,2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References
  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.
Long Name
Axl Receptor Tyrosine Kinase
Entrez Gene IDs
558 (Human); 26362 (Mouse)
Alternate Names
AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL

Product Datasheets

Citations for Human Axl APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Characterization of the Filovirus-Resistant Cell Line SH-SY5Y Reveals Redundant Role of Cell Surface Entry Factors
    Authors: FJ Zapatero-B, E Dietzel, O Dolnik, K Döhner, R Costa, B Hertel, B Veselkova, J Kirui, A Klintworth, MP Manns, S Pöhlmann, T Pietschman, T Krey, S Ciesek, G Gerold, B Sodeik, S Becker, T von Hahn
    Viruses, 2019;11(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Lipopolysaccharide inhalation recruits monocytes and dendritic cell subsets to the alveolar airspace
    Authors: L Jardine, S Wiscombe, G Reynolds, D McDonald, A Fuller, K Green, A Filby, I Forrest, MH Ruchaud-Sp, J Scott, M Collin, M Haniffa, AJ Simpson
    Nat Commun, 2019;10(1):1999.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Reviews for Human Axl APC-conjugated Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Human Axl APC-conjugated Antibody
By Anonymous on 06/03/2018
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human

the gating was based on FMO control. In my hand, Axl antibody with APC works better than PE.


Human Axl APC-conjugated Antibody
By Anonymous on 09/10/2016
Application: Flow Sample Tested: ASTROCYTE Species: Human

Human Axl APC-conjugated Antibody
By Anonymous on 07/13/2016
Application: Flow Sample Tested: Human AML cells Species: Human