Intracellular Staining by Flow Cytometry
|Detection of beta ‑Catenin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human beta ‑Catenin Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC13292F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
beta -Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved the canonical Wnt signal transduction pathways.
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