Mouse IgG2A Fluorescein-conjugated Antibody

(6 citations)
(1 Review)
  
  • Specificity
    Selected for use as mouse IgG2A isotype control for flow cytometry applications.
  • Source
    Monoclonal Mouse IgG2A Clone # 20102
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IgGControl by Flow Cytometry Human peripheral blood granulocyteswere stained with Mouse Anti-Human CXCR1/IL‑8 RA Fluorescein‑conjugatedMonoclonal Antibody
(Catalog # FAB330F,filled histogram) or Mouse IgG Fluorescein-conjugated Isotype Control Antibody(Catalog # IC0003F, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2A Fc
  • Alternate Names:
    IgG2A
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
    Authors: Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S
    PLoS ONE, 2011;6(7):e22504.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  2. Changes in chemokines and chemokine receptor expression on tonsillar B cells upon Epstein-Barr virus infection.
    Authors: Ehlin-Henriksson B, Liang W, Cagigi A, Mowafi F, Klein G, Nilsson A
    Immunology, 2009;127(4):549-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  3. CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.
    Authors: Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
    Eur. J. Immunol., 2008;38(11):3050-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  4. Sinomenine suppresses TNF-alpha-induced VCAM-1 expression in human umbilical vein endothelial cells.
    Authors: Huang J, Lin Z, Luo M, Lu C, Kim MH, Yu B, Gu J
    J Ethnopharmacol, 2007;114(2):180-5.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. HIF-1 and NF-kappaB-mediated upregulation of CXCR1 and CXCR2 expression promotes cell survival in hypoxic prostate cancer cells.
    Authors: Maxwell PJ, Gallagher R, Seaton A, Wilson C, Scullin P, Pettigrew J, Stratford IJ, Williams KJ, Johnston PG, Waugh DJ
    Oncogene, 2007;26(52):7333-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  6. Human CXCR2 (hCXCR2) takes over functionalities of its murine homolog in hCXCR2 knockin mice.
    Authors: Mihara K, Smit MJ, Krajnc-Franken M, Gossen J, Rooseboom M, Dokter W
    Eur. J. Immunol., 2005;35(9):2573-82.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 13  
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Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 2  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

Ctrl IC003G  
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Mouse IgG2A PerCP-conjugated Isotype Control

Ctrl IC003C 1
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Mouse IgG2A Alexa Fluor® 405-conjugated Isotype Control

Ctrl IC003V
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Mouse IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC003N  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC003R
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Mouse IgG2A Alexa Fluor® 594-conjugated Isotype Control

Ctrl IC003T
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Mouse IgG2A Alexa Fluor® 750-conjugated Isotype Control

Ctrl IC003S
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Mouse IgG2A Biotinylated Isotype Control

Ctrl IC003B
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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 Flow Human Anonymous 07/07/2017
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Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood cells
SpeciesHuman

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