Mouse IgG2A Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Product Details
Citations (10)
Supplemental Products
Reviews (2)

Mouse IgG2A Fluorescein-conjugated Antibody Summary

Selected for use as mouse IgG2A isotype control for flow cytometry applications.
Monoclonal Mouse IgG2A Clone # 20102
Protein A or G purified from hybridoma culture supernatant
Supplied in a saline solution containing BSA and Sodium Azide.
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Detection of Mouse IgG Control by Flow Cytometry Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB330F, filled histogram) or Mouse IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC0003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names

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Citations for Mouse IgG2A Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Leptin-producing monocytes in the airway submucosa may contribute to asthma pathogenesis
    Authors: K Watanabe, M Suzukawa, S Kawauchi-W, S Igarashi, I Asari, S Imoto, H Tashimo, T Fukami, A Hebisawa, S Tohma, T Nagase, K Ohta
    Respiratory investigation, 2022-11-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
  2. Interleukin-6 Signaling in Triple Negative Breast Cancer Cells Elicits the Annexin A1/Formyl Peptide Receptor 1 Axis and Affects the Tumor Microenvironment
    Authors: L Vecchi, STS Mota, MAP Zóia, IC Martins, JB de Souza, TG Santos, AO Beserra, VP de Andrade, LR Goulart, TG Araújo
    Cells, 2022-05-20;11(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  3. Annexin A1 promotes the nuclear localization of the epidermal growth factor receptor in castration-resistant prostate cancer
    Authors: STS Mota, L Vecchi, DA Alves, AO Cordeiro, GS Guimarães, E Campos-Fer, YCP Maia, BC Dornelas, SM Bezerra, VP de Andrade, LR Goulart, TG Araújo
    Int. J. Biochem. Cell Biol., 2020-08-25;127(0):105838.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  4. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation
    Authors: JM Laufer, MA Hauser, I Kindinger, V Purvanov, A Pauli, DF Legler
    Cell Rep, 2019-10-22;29(4):995-1009.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
    Authors: Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S
    PLoS ONE, 2011-07-25;6(7):e22504.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  6. Changes in chemokines and chemokine receptor expression on tonsillar B cells upon Epstein-Barr virus infection.
    Authors: Ehlin-Henriksson B, Liang W, Cagigi A, Mowafi F, Klein G, Nilsson A
    Immunology, 2009-08-01;127(4):549-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  7. CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.
    Authors: Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
    Eur. J. Immunol., 2008-11-01;38(11):3050-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  8. Sinomenine suppresses TNF-alpha-induced VCAM-1 expression in human umbilical vein endothelial cells.
    Authors: Huang J, Lin Z, Luo M, Lu C, Kim MH, Yu B, Gu J
    J Ethnopharmacol, 2007-08-11;114(2):180-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. HIF-1 and NF-kappaB-mediated upregulation of CXCR1 and CXCR2 expression promotes cell survival in hypoxic prostate cancer cells.
    Authors: Maxwell PJ, Gallagher R, Seaton A, Wilson C, Scullin P, Pettigrew J, Stratford IJ, Williams KJ, Johnston PG, Waugh DJ
    Oncogene, 2007-05-28;26(52):7333-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  10. Human CXCR2 (hCXCR2) takes over functionalities of its murine homolog in hCXCR2 knockin mice.
    Authors: Mihara K, Smit MJ, Krajnc-Franken M, Gossen J, Rooseboom M, Dokter W
    Eur. J. Immunol., 2005-09-01;35(9):2573-82.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control


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Reviews for Mouse IgG2A Fluorescein-conjugated Antibody

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Mouse IgG2A Fluorescein-conjugated Antibody
By Anonymous on 02/02/2023
Application: Flow Sample Tested: 293T human embryonic kidney cell line Species: Human

Use as Iso-type Ctrl

Mouse IgG2A Fluorescein-conjugated Antibody
By Anonymous on 07/07/2017
Application: Flow Sample Tested: Peripheral blood cells Species: Human