Human Cadherin‑7 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # FAB11591AFP488

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 1087216
View all Cadherin-7 Primary Antibodies »

Product Specifications

Specificity

Detects rhCDH-7 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Cadherin‑7 Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cadherin-7

Cadherin-7 is an approximately 115 kDa type I transmembrane protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Human Cadherin-7 is synthesized with a 27 amino acid (aa) signal peptide and a 20 aa N-terminal propeptide. The mature cell surface-expressed protein consists of a 738 amino acid (aa) extracellular domain (ECD) that contains five Cadherin repeats, a 21 aa transmembrane segment, and a 157 aa cytoplasmic domain (2, 3). Within the ECD, human Cadherin-7 shares 99% and 97% aa sequence identity with mouse and rat Cadherin-7, respectively. Cadherin-7 interacts homotypically and heterotypically with Cadherin-14 and more weakly with Cadherins-6, -9, and -12 (3, 4). Cellular adhesion mediated by Cadherin-7 is more weak than that mediated by E- or N-Cadherin, although it can be strengthed by Fibronectin binding to Integrins on the same cell (5, 6). Cadherin-7 is localized to discrete regions of the developing nervous system. In chick and mouse, it is expressed in the basal plate of the neural tube, migrating cranial motoneurons, and migrating neural crest cells (4, 7, 8), lateral regions of the hindbrain and migrating Purkinje cell precursors (8, 9), striatum, parahippocampal areas, and somatosensory cortex of the forebrain (10 - 12), neural retina and cochlea (13, 14). Cadherin-7 interactions promote motor axon growth and inhibit axonal branching, whereas Cadherin-6B promotes branching during cranial motorneuron development (7). Cadherin-7 performs a similar function during limb bud development during which it participates in the migration and condensation of mesenchymal cells (15). Cadherin-7 is overexpressed on primary melanoma cells where it binds melanoma inhibitory activity (MIA), a secreted melanoma cell protein that promotes tumor progression (16).

References

  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Kools, P. et al. (2000) Genomics 68:283.
  3. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
  4. Nakagawa, S. et al. (1995) Development 121:1321.
  5. Chu, Y.-S. et al. (2006) J. Biol. Chem. 281:2901.
  6. Martinez-Rico, C. et al. (2009) J. Cell Sci. 123:712.
  7. Barnes, S.H. et al. (2010) Development 137:805.
  8. Ju, M.J. et al. (2004) Neuroscience 128:785.
  9. Luo, J. et al. (2004) Mol. Cell. Neurosci. 25:138.
  10. Redies, C. et al. (2002) Brain Res. Bull. 57:489.
  11. Kovjanic, D. and C. Redies (2003) J. Comp. Neurol. 456:95.
  12. Krishna-K, K. et al. (2011) Neuroscience 175:37.
  13. Faulkner-Jones, B.E. et al. (1999) Mol. Cell. Neurosci. 14:1.
  14. Luo, J. et al. (2007) Dev. Dyn. 236:2331.
  15. Kim, D. et al. (2009) J. Cell. Physiol. 221:161.
  16. Winklmeier, A. et al. (2009) Cancer Sci. 100:261.

Alternate Names

CAD7, Cadherin7, CDH7, CDH7L1

Entrez Gene IDs

1005 (Human); 241201 (Mouse); 29162 (Rat)

Gene Symbol

CDH7

UniProt

Q9ULB5 (Human)

Additional Cadherin-7 Products

Product Documents for Human Cadherin‑7 Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Cadherin‑7 Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

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Protocols

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