Human Calreticulin-2 Alexa Fluor® 350-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Calreticulin-2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Calreticulin is observed.
  • Source
    Monoclonal Mouse IgG2b Clone # 321007
  • Immunogen
    E. coli-derived recombinant human Calreticulin-2
    Thr20-Leu384
    Accession # Q96L12
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 350
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    MCF-7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Calreticulin-2
Calreticulin-2 is a soluble lectin-like molecule that participates in the protein folding and quality control cycle in the endoplasmic reticulum (ER) lumen. Mature human Calreticulin-2 has a 178 amino acid N-terminal globular domain, a 97 aa central P-domain, and a 90 aa C-terminal domain. The globular domain shows lectin activity, the P-domain binds one Ca++ ion with high affinity, and the C-terminus binds Ca++ and contains an RNEL ER retention motif. The amino acid sequence of Calreticulin‑2 is 84%, 83% and 87% identical to that of mouse, bovine and canine Calreticulin‑2, respectively.
  • Entrez Gene IDs:
    125972 (Human); 73316 (Mouse); 364529 (Rat)
  • Alternate Names:
    CALR3; calreticulin 2; calreticulin 3; Calreticulin2; Calreticulin-2; calreticulin-3; cancer/testis antigen 93; Crt2; CRT2calreticulin-2; CT93; FLJ25355; MGC26577
Related Research Areas

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