Human Calreticulin-2 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC2927G
Detection of Calreticulin‑2 in MCF‑7 Human Cell Line by Flow Cytometry.
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Human Calreticulin-2 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Calreticulin-2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Calreticulin is observed.
Source
Monoclonal Mouse IgG2B Clone # 321007
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Calreticulin-2
Thr20-Leu384
Accession # Q96L12
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Calreticulin‑2 in MCF‑7 Human Cell Line by Flow Cytometry. View Larger

Detection of Calreticulin‑2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Calreticulin‑2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC2927G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Calreticulin-2

Calreticulin-2 is a soluble lectin-like molecule that participates in the protein folding and quality control cycle in the endoplasmic reticulum (ER) lumen. Mature human Calreticulin-2 has a 178 amino acid N-terminal globular domain, a 97 aa central P-domain, and a 90 aa C-terminal domain. The globular domain shows lectin activity, the P-domain binds one Ca++ ion with high affinity, and the C-terminus binds Ca++ and contains an RNEL ER retention motif. The amino acid sequence of Calreticulin‑2 is 84%, 83% and 87% identical to that of mouse, bovine and canine Calreticulin‑2, respectively.

Entrez Gene IDs
125972 (Human); 73316 (Mouse); 364529 (Rat)
Alternate Names
CALR3; calreticulin 2; calreticulin 3; Calreticulin2; Calreticulin-2; calreticulin-3; cancer/testis antigen 93; Crt2; CRT2calreticulin-2; CT93; FLJ25355; MGC26577

Product Datasheets

FAQs

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