Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Carbonic Anhydrase I/CA1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase I/CA1
Ala2-Phe261
Accession # P00915

Specificity

Detects human Carbonic Anhydrase I/CA1 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) CA2, rhCA3, rhCA4, rhCA8, rhCA9, rhCA10, rhCA12, rhCA13, and rhCA14 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Carbonic Anhydrase I/CA1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Carbonic Anhydrase I (Catalog # 2180-CA)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Carbonic Anhydrase I/CA1

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation (2). The activity of CA1 can also be measured by its ability to catalyze the reaction CO2 + H2O → HCO3- + H+, using a published method (3).

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.
  3. Wilbur, K.M. and N.G. Anderson (1948) J. Biol. Chem. 176:147.

Alternate Names

CA1

Entrez Gene IDs

759 (Human)

Gene Symbol

CA1

UniProt

P00915

Additional Carbonic Anhydrase I/CA1 Products

Product Documents for Human Carbonic Anhydrase I/CA1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Carbonic Anhydrase I/CA1 Biotinylated Antibody

For research use only

Customer Reviews for Human Carbonic Anhydrase I/CA1 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human Carbonic Anhydrase I/CA1 Biotinylated Antibody and earn rewards!

Have you used Human Carbonic Anhydrase I/CA1 Biotinylated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies