Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Carbonic Anhydrase I/CA1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase I/CA1
Ala2-Phe261
Accession # P00915

Specificity

Detects human Carbonic Anhydrase I/CA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) CA2, rhCA3, rhCA4, rhCA8, rhCA9, rhCA10, and rhCA12 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Carbonic Anhydrase I/CA1 Antibody

Detection of Human Carbonic Anhydrase I/CA1 antibody by Western Blot.

Detection of Human Carbonic Anhydrase I/CA1 by Western Blot.

Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, human liver tissue, and human colon tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Carbonic Anhydrase I/CA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Carbonic Anhydrase I/CA1 antibody by Simple WesternTM.

Detection of Human Carbonic Anhydrase I/CA1 by Simple WesternTM.

Simple Western lane view shows lysates of HEL 92.1.7 human erythroleukemic cell line and human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 38 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Carbonic Anhydrase I/CA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2180) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Carbonic Anhydrase I/CA1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase I (Catalog # 2180-CA), see our available Western blot detection antibodies

Simple Western

5 µg/mL
Sample: HEL 92.1.7 human erythroleukemic cell line and human liver tissue

Western Blot

0.1 µg/mL
Sample: HEL 92.1.7 human erythroleukemic cell line, human liver tissue, and human colon tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase I/CA1

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation (2). The activity of CA1 can also be measured by its ability to catalyze the reaction CO2 + H2O → HCO3- + H+, using a published method (3).

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.
  3. Wilbur, K.M. and N.G. Anderson (1948) J. Biol. Chem. 176:147.

Alternate Names

CA1

Entrez Gene IDs

759 (Human)

Gene Symbol

CA1

UniProt

P00915

Additional Carbonic Anhydrase I/CA1 Products

Product Documents for Human Carbonic Anhydrase I/CA1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Carbonic Anhydrase I/CA1 Antibody

For research use only

Citations for Human Carbonic Anhydrase I/CA1 Antibody

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