|Detection of Human Carbonic Anhydrase I/CA1 by Western Blot. Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, human liver tissue, and human colon tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Carbonic Anhydrase I/CA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Detection of Human Carbonic Anhydrase I/CA1 by Simple WesternTM. Simple Western lane view shows lysates of HEL 92.1.7 human erythroleukemic cell line and human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 38 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Carbonic Anhydrase I/CA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2180) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.|
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation (2). The activity of CA1 can also be measured by its ability to catalyze the reaction CO2 + H2O → HCO3- + H+, using a published method (3).
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