Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Carbonic Anhydrase XIV/CA14 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XIV/CA14
Gly19-Met290
Accession # Q9ULX7

Specificity

Detects human Carbonic Anhydrase XIV/CA14 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CA14 is observed and less than 5% cross-reactivity with recombinant human (rh) CA1, rhCA2, rhCA3, rhCA4, rhCA8, rhCA9, rhCA10, rhCA12, and rhCA13 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Carbonic Anhydrase XIV/CA14 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Carbonic Anhydrase XIV (Catalog # 2195-CA)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase XIV/CA14

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as anutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA14 is a type I membrane enzyme highly expressed in all parts of the central nervous system with lower expression in adult liver, heart, small intestine, colon, kidney, urinary bladder, and skeletal muscle (2).

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Fujikawa-Adachi, K. et al. (1999) Genomics 61:74.

Alternate Names

CA14

Entrez Gene IDs

23632 (Human); 23831 (Mouse)

Gene Symbol

CA14

UniProt

Q9ULX7

Additional Carbonic Anhydrase XIV/CA14 Products

Product Documents for Human Carbonic Anhydrase XIV/CA14 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Carbonic Anhydrase XIV/CA14 Biotinylated Antibody

For research use only

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