Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Carbonic Anhydrase XIV/CA14 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XIV/CA14
Gly19-Met290
Accession # Q9ULX7

Specificity

Detects human Carbonic Anhydrase XIV/CA14 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Carbonic Anhydrase XIV/CA14 Antibody

Detection of Human and Mouse Carbonic Anhydrase XIV/CA14 antibody by Western Blot.

Detection of Human and Mouse Carbonic Anhydrase XIV/CA14 by Western Blot.

Western blot shows lysates of human and mouse brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Carbonic Anhydrase XIV/CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2195) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse Carbonic Anhydrase XIV/CA14 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase XIV (Catalog # 2195-CA), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Human and mouse brain (cerebellum) tissue

Reviewed Applications

Read 1 review rated 5 using AF2195 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase XIV/CA14

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as anutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA14 is a type I membrane enzyme highly expressed in all parts of the central nervous system with lower expression in adult liver, heart, small intestine, colon, kidney, urinary bladder, and skeletal muscle (2).

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Fujikawa-Adachi, K. et al. (1999) Genomics 61:74.

Alternate Names

CA14

Entrez Gene IDs

23632 (Human); 23831 (Mouse)

Gene Symbol

CA14

UniProt

Q9ULX7

Additional Carbonic Anhydrase XIV/CA14 Products

Product Documents for Human/Mouse Carbonic Anhydrase XIV/CA14 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Carbonic Anhydrase XIV/CA14 Antibody

For research use only

Customer Reviews for Human/Mouse Carbonic Anhydrase XIV/CA14 Antibody (1)

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  • Human/Mouse Carbonic Anhydrase XIV/CA14 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: melanoma cell lysates of human and mouse
    Species: human and mouse
    Verified Customer | Posted 04/21/2026
    Whole cell lysates were blotted with 1o CA14 goat polyclonal antibody AF2195 and then 2o anti-Goat HRP. The results are clean without any non-specific bands.
    Human/Mouse Carbonic Anhydrase XIV/CA14 Antibody AF2195

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