Human CCL13/MCP-4 APC-conjugated Antibody

Name: Human CCL13/MCP-4 APC-conjugated Antibody
Brand: R&D Systems
SKU: IC327A

Catalog #: IC327A Datasheet / COA / SDS

Discontinued Product

IC327A has been discontinued.

View all CCL13/MCP-4 products.

Detection of CCL13/MCP‑4 in HT‑29 Human Cell Line by Flow Cytometry.

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All CCL13/MCP-4 Antibodies

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Human CCL13/MCP-4 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL13/MCP‑4 in ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 73506

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human CCL13/MCP-4
Gln24-Thr98
Accession # Q99616.1

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of CCL13/MCP-4 antibody in HT-29 Human Cell Line antibody by Flow Cytometry.

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Detection of CCL13/MCP‑4 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human CCL13/MCP-4 APC-conjugated Monoclonal Antibody (Catalog # IC327A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCL13/MCP-4

CCL13, also known as Monocyte Chemoattractant Protein-4 (MCP-4), is a CC chemokine that acts as a chemoattractant for monocytes, eosinophils and T cells and as an activator of basophils. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. The bioactivities of CCL13 are mediated by the CC chemokine receptors CCR-2 and CCR-3.

Entrez Gene IDs

6357 (Human)

Alternate Names

C-C motif chemokine 13; CCL13; chemokine (C-C motif) ligand 13; CKb10; MCP4; MCP-4; MCP-4Monocyte chemoattractant protein 4; MGC17134; NCC-1Monocyte chemotactic protein 4; NCC1Small-inducible cytokine A13; new CC chemokine 1; SCYA13CK-beta-10; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13