Intracellular Staining by Flow Cytometry
|Detection of CCL19/MIP‑3 beta in Human Monocyte-derived Dendritic Cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Mouse Anti-Human CCL19/MIP‑3 beta PE‑conjugated Monoclonal Antibody (Catalog # IC361P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
CCL19, also known as MIP-3 beta and ELC (EBI1-Ligand Chemokine), is a 77 amino acid (aa) beta chemokine that is distantly related to other beta chemokines (20-30% aa sequence identity). The gene for MIP-3 beta has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta is constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is down‑regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta is chemotactic for cultured human lymphocytes. MIP-3 beta is a ligand for CCR7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.
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