Human CCL19/MIP‑3 beta PE‑conjugated Antibody

R&D Systems | Catalog # IC361P

R&D Systems
Discontinued Product
IC361P has been discontinued. View all CCL19/MIP-3 beta products.

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 54909
View all CCL19/MIP-3 beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL19/MIP-3 beta
Gly22-Ser98
Accession # Q99731.1

Specificity

Detects human CCL19/MIP-3 beta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 25, recombinant mouse CCL2, 3, 4, 5, 6, 7, 9, 11, 12, 21, 22, or 25 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CCL19/MIP‑3 beta PE‑conjugated Antibody

Detection of CCL19/MIP‑3 beta antibody in Human Monocyte-derived Dendritic Cells antibody by Flow Cytometry.

Detection of CCL19/MIP‑3 beta in Human Monocyte-derived Dendritic Cells by Flow Cytometry.

Human monocyte-derived dendritic cells were stained with Mouse Anti-Human CCL19/MIP-3 beta PE-conjugated Monoclonal Antibody (Catalog # IC361P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human CCL19/MIP‑3 beta PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human monocyte-derived dendritic cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCL19/MIP-3 beta

CCL19, also known as MIP-3 beta and ELC (EBI1-Ligand Chemokine), is a 77 amino acid (aa) beta chemokine that is distantly related to other beta chemokines (20-30% aa sequence identity). The gene for MIP-3 beta has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta is constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is down‑regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta is chemotactic for cultured human lymphocytes. MIP-3 beta is a ligand for CCR7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.

References

  1. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

Alternate Names

ELC, MIP-3 beta, MIP3 beta

Entrez Gene IDs

6363 (Human); 24047 (Mouse); 362506 (Rat)

Gene Symbol

CCL19

UniProt

Q99731.1

Additional CCL19/MIP-3 beta Products

Product Documents for Human CCL19/MIP‑3 beta PE‑conjugated Antibody

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Product Specific Notices for Human CCL19/MIP‑3 beta PE‑conjugated Antibody

For research use only

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