Human CCL2/JE/MCP-1 Alexa Fluor® 647-conjugated Antibody

• Species Reactivity
  Human
• Specificity
  Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human CCL1, 3, 4, 5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9,10, MIP-1 gamma, 11, 12, 17, 19, 20, 22, 24, 25, or recombinant rat CCL20.
• Source
  Monoclonal Mouse IgG₁ Clone # 24822
• Purification
  Protein A or G purified from ascites
• Immunogen
  E. coli-derived recombinant human CCL2/JE/MCP-1
  Accession # P13500
• Formulation
  Supplied in a saline solution containing BSA and Sodium Azide.
• Label
  Alexa Fluor 647

• Intracellular Staining by Flow Cytometry
  5 µL/10⁶ cells
  See below

Intracellular Staining by Flow Cytometry click image to view larger

Detection of CCL2/JE/MCP‑1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 250 ng/mL LPS overnight or (B) untreated were stained with Mouse Anti-Human CCL2/JE/MCP‑1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC279R) and Mouse Anti-Human CD11c PE‑conjugated Monoclonal Antibody (Catalog # FAB1777P). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Shipping
  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
• Stability & Storage
  Protect from light. Do not freeze.

  • 12 months from date of receipt, 2 to 8 °C as supplied.

• Entrez Gene IDs:
  6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

• Alternate Names:
  C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

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