Human CCL2/JE/MCP-1 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
IC279R-025
Detection of CCL2/JE/MCP‑1 in Human PBMCs by Flow Cytometry.
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Human CCL2/JE/MCP-1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human CCL1, 3, 4, 5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9,10, MIP-1 gamma, 11, 12, 17, 19, 20, 22, 24, 25, or recombinant rat CCL20.
Source
Monoclonal Mouse IgG1 Clone # 24822
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL2/JE/MCP-1
Accession # P13500
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of CCL2/JE/MCP‑1 in Human PBMCs by Flow Cytometry. View Larger

Detection of CCL2/JE/MCP‑1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 250 ng/mL LPS overnight or (B) untreated were stained with Mouse Anti-Human CCL2/JE/MCP‑1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC279R) and Mouse Anti-Human CD11c PE‑conjugated Monoclonal Antibody (Catalog # FAB1777P). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCL2/JE/MCP-1

CCL2 is a beta -chemokine that is also known as Monocyte Chemotactic Protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes and basophils. MCP-1 exists in equilibrium as either a monomer or homodimer, and forms a heterodimer with multiple chemokines. Human and mouse CCL2 share 58% amino acid sequence identity.

Entrez Gene IDs
6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

Product Datasheets

FAQs

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