Human CCL2/JE/MCP-1 Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry View Larger

Detection of CCL2/JE/MCP‑1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 250 ng/mL LPS overnight or (B) untreated were stained with Mouse Anti-Human CCL2/JE/MCP‑1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC279R) and Mouse Anti-Human CD11c PE‑conjugated Monoclonal Antibody (Catalog # FAB1777P). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Background: CCL2/JE/MCP-1

CCL2 is a beta -chemokine that is also known as Monocyte Chemotactic Protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes and basophils. MCP-1 exists in equilibrium as either a monomer or homodimer, and forms a heterodimer with multiple chemokines. Human and mouse CCL2 share 58% amino acid sequence identity.

Product Datasheets

Related Research Areas

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• Chemokines and Receptors in Allergy and Asthma
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• gamma delta T Cells
• HIF Transcription Factors
• Inflammatory Mediators
• Macrophage Activation Markers
• Molecules Secreted by VSMC
• Neuroinflammation
• Uptake of Amyloid beta by Macrophages