Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with other chemokines, including recombinant human CCL2, 3, 4, recombinant mouse (rm) CCL3, and rmCCL4.
Monoclonal Mouse IgG1 Clone # 21445
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CCL5/RANTES Accession # P13501
Supplied in a saline solution containing BSA and Sodium Azide.
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CCL5/RANTES in NS0 Mouse Cell Line Transfected with Human CCL5/RANTES by Flow Cytometry. NS0 mouse myeloma cell line transfected with human CCL5/RANTES was stained with Mouse Anti-Human CCL5/RANTES PE‑conjugated Monoclonal Antibody (Catalog # IC278P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily and is now designated CCL5. It binds and activates the chemokine receptors CCR1, 3 and 5, and is reported to be a ligand for GPR75. CCL5 contains O-linked glycosylation and undergoes N-terminal processing by MMP-13. CCL5 homooligomers likely exist, and heterodimers with CXCL4 and CCL2 have been noted. Functionally, CCL5 promotes the migration of CD8+ T cells, macrophages, and eosinophils, and induces the formation of chemokine-activated killer cells. Human and mouse CCL5 share 84% sequence identity.
chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.