Human CCL5/RANTES PE-conjugated Antibody

IC278P has been discontinued. View all CCL5/RANTES products.
(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with other chemokines, including recombinant human CCL2, 3, 4,  recombinant mouse (rm) CCL3, and rmCCL4.
  • Source
    Monoclonal Mouse IgG1 Clone # 21445
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human CCL5/RANTES
    Accession # P13501
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of CCL5/RANTES in NS0 Mouse Cell Line Transfected with Human CCL5/RANTES by Flow Cytometry. NS0 mouse myeloma cell line transfected with human CCL5/RANTES was stained with Mouse Anti-Human CCL5/RANTES PE‑conjugated Monoclonal Antibody (Catalog # IC278P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCL5/RANTES
RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily and is now designated CCL5. It binds and activates the chemokine receptors CCR1, 3 and 5, and is reported to be a ligand for GPR75. CCL5 contains O-linked glycosylation and undergoes N-terminal processing by MMP-13. CCL5 homooligomers likely exist, and heterodimers with CXCL4 and CCL2 have been noted. Functionally, CCL5 promotes the migration of CD8+ T cells, macrophages, and eosinophils, and induces the formation of chemokine-activated killer cells. Human and mouse CCL5 share 84% sequence identity.
  • Entrez Gene IDs:
    6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
  • Alternate Names:
    CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Cooperative phagocytes: resident microglia and bone marrow immigrants remove dead photoreceptors in retinal lesions.
    Authors: Joly S, Francke M, Ulbricht E, Beck S, Seeliger M, Hirrlinger P, Hirrlinger J, Lang KS, Zinkernagel M, Odermatt B, Samardzija M, Reichenbach A, Grimm C, Reme CE
    Am. J. Pathol., 2009;174(6):2310-23.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes.
    Authors: Nobile C, Lind M, Miro F, Chemin K, Tourret M, Occhipinti G, Dogniaux S, Amigorena S, Hivroz C
    Blood, 2008;111(7):3579-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. NF-kappaB modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gammadelta lymphocytes.
    Authors: Ferrarini M, Delfanti F, Gianolini M, Rizzi C, Alfano M, Lazzarin A, Biswas P
    J. Immunol., 2008;181(9):5857-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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