Human CCR10 APC-conjugated Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detect human CCR10. Stains human CCR10-transfected cells but not irrelevant transfectants.
  • Source
    Monoclonal Rat IgG2A Clone # 314305
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Y3 rat myeloid cell line transfected with human CCR10
    Gln8-Asn362
    Accession # P46092
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR10 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB100F) and either (A) Rat Anti-Human CCR10 APC‑conjugated Monoclonal Antibody (Catalog # FAB3478A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR10 in L1.2 Mouse Cell Line Transfected with Human CCR10 and eGFP by Flow Cytometry. L1.2 mouse pro-B cell line transfected with human CCR10 and eGFP was stained with either (A) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A) or (B) Rat Anti-Human CCR10 APC‑conjugated Monoclonal Antibody (Catalog # FAB3478A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27 and CCL28. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression is correlated with the metastatic behavior of melanomas.

  • Entrez Gene IDs:
    2826 (Human); 12777 (Mouse); 363682 (Rat)
  • Alternate Names:
    CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. A role for CCL28-CCR3 in T-cell homing to the human upper airway mucosa.
    Authors: Danilova, E, Skrindo, I, Gran, E, Hales, B J, Smith, W A, Jahnsen, J, Johansen, F E, Jahnsen, F L, Baekkevold, E S
    Mucosal Immunol, 2015;8(1):107-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Plasmablasts as migratory IgG-producing cells in the pathogenesis of neuromyelitis optica.
    Authors: Chihara, Norio, Aranami, Toshimas, Oki, Shinji, Matsuoka, Takako, Nakamura, Masakazu, Kishida, Hitaru, Yokoyama, Kazumasa, Kuroiwa, Yoshiyuk, Hattori, Nobutaka, Okamoto, Tomoko, Murata, Miho, Toda, Tatsushi, Miyake, Sachiko, Yamamura, Takashi
    PLoS ONE, 2013;8(12):e83036.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab.
    Authors: Mei HE, Frolich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, Feist E, Daridon C, Tony HP, Radbruch A, Dorner T
    Blood, 2010;116(24):5181-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. CCL28 is increased in human Helicobacter pylori-induced gastritis and mediates recruitment of gastric immunoglobulin A-secreting cells.
    Authors: Hansson M, Hermansson M, Svensson H, Elfvin A, Hansson LE, Johnsson E, Sjoling A, Quiding-Jarbrink M
    Infect. Immun., 2008;76(7):3304-11.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 7  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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