Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-reported

Cited:

Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 314305
View all CCR10 Primary Antibodies »

Product Specifications

Immunogen

Y3 rat myeloid cell line transfected with human CCR10
Gln8-Asn362
Accession # P46092

Specificity

Detect human CCR10. Stains human CCR10-transfected cells but not irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human CCR10 Antibody

Detection of CCR10 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CCR10 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Rat Anti-Human CCR10 Monoclonal Antibody (Catalog # MAB3478) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Detection of Human CCR10/GPR2 by Flow Cytometry

Detection of Human CCR10/GPR2 by Flow Cytometry

The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells.Expression of EBNA-2 and LMP-1 in IL-21 treated cells (A, B). (A) Simultaneous immunofluorescence staining of EBNA-2 (Green) and LMP-1 (Red); magnification (×100), scale bar 25 µm. Note the downregulation of EBNA-2 and upregultion of LMP-1 after IL-21 treatment. (B) Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5×105 cells were loaded in the control lanes and 5×105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. (C) Activity of the W and C promoters that regulate EBNA-2 expression and LMP-1 mRNA expression by Q-PCR. Note the difference in EBNA-2 regulation; the MEC2 cell uses both Wp and Cp while in MEC1 only Wp is active. (D) Expression of EBNA-2 and LMP-1 in cells exposed to CD40L. Simultaneous immunofluorescence staining; for details see (A). Note: EBNA-2 and LMP-1 are downregulated by CD40L in both lines. (E) CD40L induced modulation of surface marker by FACS analysis. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CCR10/GPR2 by Flow Cytometry

Detection of Human CCR10/GPR2 by Flow Cytometry

Comparison of the MEC1 and MEC2 cells.(A) Expression of EBV encoded proteins EBNA-2 and LMP-1 by immunofluorescence; magnification (×100), scale bar 25 µm. Note: the MEC2 cells are larger. (B) Expression of EBNA-2 and LMP-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5×105 cells were loaded in control lanes and 5×105 were loaded in MEC1 and MEC2 lanes. Note MEC2 expresses higher amount of EBNA-2. (C) Expression of Bright and BARF1 by Q-PCR. (D) FACS analysis of surface markers that are differently expressed in the 2 lines. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CCR10 Antibody

Application
Recommended Usage

CyTOF-reported

This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample:

Human peripheral blood lymphocytes

Reviewed Applications

Read 1 review rated 4 using MAB3478 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that function as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Alternate Names

CCR10, GPR2

Entrez Gene IDs

2826 (Human); 12777 (Mouse); 363682 (Rat)

Gene Symbol

CCR10

UniProt

P46092

Additional CCR10 Products

Product Documents for Human CCR10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCR10 Antibody

For research use only

Citations for Human CCR10 Antibody

Customer Reviews for Human CCR10 Antibody (1)

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  • Human CCR10 Antibody
    Name: Anonymous
    Application: CyTOF
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 06/28/2016
    Human CCR10 Antibody MAB3478

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