Human CCR5 PE-conjugated Antibody

  (6 citations)
(2 Reviews)
    
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human CCR5.
  • Source
    Monoclonal Mouse IgG2B Clone # 45531
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CCR5
    Met1-Leu352
    Accession # P51681
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CCR5 in NS0 Mouse Cell Line Transfected with Human CCR5 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human CCR5 was stained with Mouse Anti-Human CCR5 PE‑conjugated Monoclonal Antibody (Catalog # FAB182P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR5
CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for macrophage tropic HIV-1 infection.
  • Entrez Gene IDs:
    1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
  • Alternate Names:
    C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Human TCR gamma delta+ T cells represent a novel target for IL-27 activity.
    Authors: Morandi F, Prigione I, Airoldi I
    Eur. J. Immunol., 2012;42(6):1547-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
    Authors: Badr G, Lefevre EA, Mohany M
    PLoS ONE, 2011;6(9):e23741.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.
    Authors: Brucklacher-Waldert V, Steinbach K, Lioznov M, Kolster M, Holscher C, Tolosa E
    J. Immunol., 2009;183(9):5494-501.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
    Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
    Int. J. Cancer, 2006;118(9):2147-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 24  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 2 reviews)

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We have 2 review tested in 1 species: Human.
We have 2 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Flow Human Anonymous 12/11/2017
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Summary

Application Flow Cytometry
Sample Tested Blood mononuclear cells (PBMCs)
Species Human
Flow Cytometry Human CCR5 PE-conjugated Antibody FAB182P Array FAB182P
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Excellent
 Flow Human Anonymous 06/28/2017
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Image Details
Flow Cytometry Human CCR5 PE-conjugated Antibody FAB182P
Flow Cytometry: Human CCR5 PE-conjugated Antibody [FAB182P].

Summary

Application Flow Cytometry
Sample Tested U937 human histiocytic lymphoma cell line
Species Human

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