Human CCR5 Antibody

Catalog # Availability Size / Price Qty
MAB182-100
MAB182-SP
Detection of CCR5 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (28)
FAQs
Supplemental Products
Reviews (2)

Human CCR5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR5.
Source
Monoclonal Mouse IgG2B Clone # 45531
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CCR5
Met1-Leu352
Accession # P51681
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 40 ng/mL Recombinant Human CCL3/MIP‑1 alpha isoform LD78a.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CCR5 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CCR5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB182) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CCR5 antibody in Human Dendritic Cells by Immunocytochemistry (ICC). View Larger

CCR5 in Human Dendritic Cells. CCR5 was detected in immersion fixed human dendritic cells using Human CCR5 Monoclonal Antibody (Catalog # MAB182) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Chemotaxis Induced by CCL3/MIP‑1 alpha  and Neutraliza-tion by Human CCR5 Antibody. View Larger

Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutraliza-tion by Human CCR5 Antibody. Recombinant Human CCL3/MIP‑1a (Catalog # 270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL3/MIP‑1a (40 ng/mL) is neutralized (green line) by increasing concentrations of Human CCR5 Monoclonal Antibody (Catalog # MAB182). The ND50 is typically 5-20 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for macrophage tropic HIV-1 infection.

Entrez Gene IDs
1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
Alternate Names
C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5

Product Datasheets

Citations for Human CCR5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Identifying CCR5 coreceptor populations permissive for HIV-1 entry and productive infection: implications for in vivo studies
    Authors: M Weichseldo, Y Tagaya, M Reitz, AL DeVico, OS Latinovic
    Journal of Translational Medicine, 2022;20(1):39.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Modeling of CCR5 Recognition by HIV-1 gp120: How the Viral Protein Exploits the Conformational Plasticity of the Coreceptor
    Authors: C Jacquemard, F Koensgen, P Colin, B Lagane, E Kellenberg
    Viruses, 2021;13(7):.
    Species: Human
    Sample Types: Membranes
    Applications: Flow Cytometry
  3. Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells
    Authors: MC Mladinich, JN Conde, WR Schutt, SY Sohn, ER Mackow
    MBio, 2021;12(4):e0196221.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma
    Authors: YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang
    Nature Communications, 2021;12(1):4091.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5
    Authors: G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai
    Cell Death Dis, 2019;10(4):264.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. CCR5 structural plasticity shapes HIV-1 phenotypic properties
    Authors: P Colin, Z Zhou, I Staropoli, J Garcia-Per, R Gasser, M Armani-Tou, Y Benureau, N Gonzalez, J Jin, BJ Connell, S Raymond, P Delobel, J Izopet, H Lortat-Jac, J Alcami, F Arenzana-S, A Brelot, B Lagane
    PLoS Pathog., 2018;14(12):e1007432.
    Species: Human
    Sample Types: Whole Cells
    Applications: Competitive Binding Assay
  7. The HIV co-receptor CCR5 regulates osteoclast function
    Authors: JW Lee, A Hoshino, K Inoue, T Saitou, S Uehara, Y Kobayashi, S Ueha, K Matsushima, A Yamaguchi, Y Imai, T Iimura
    Nat Commun, 2017;8(1):2226.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  8. ?20 IFITM2 differentially restricts X4 and R5 HIV-1
    Authors: WL Wu, CR Grotefend, MT Tsai, YL Wang, V Radic, H Eoh, IC Huang
    Proc. Natl. Acad. Sci. U.S.A., 2017;114(27):7112-7117.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer.
    Authors: Ward, S T, Li, K K, Hepburn, E, Weston, C J, Curbishley, S M, Reynolds, G M, Hejmadi, R K, Bicknell, R, Eksteen, B, Ismail, T, Rot, A, Adams, D H
    Br J Cancer, 2015;112(2):319-28.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. CCR5 conformations are dynamic and modulated by localization, trafficking and G protein association.
    Authors: Flegler A, Cianci G, Hope T
    PLoS ONE, 2014;9(2):e89056.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  11. Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.
    Authors: Hartmann N, Giese N, Giese T, Poschke I, Offringa R, Werner J, Ryschich E
    Clin Cancer Res, 2014;20(13):3422-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
    Authors: Alonzo, Francis, Kozhaya, Lina, Rawlings, Stephen, Reyes-Robles, Tamara, DuMont, Ashley L, Myszka, David G, Landau, Nathanie, Unutmaz, Derya, Torres, Victor J
    Nature, 2013;493(7430):51-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Functional diversity of human vaginal APC subsets in directing T-cell responses.
    Authors: Duluc, D, Gannevat, J, Anguiano, E, Zurawski, S, Carley, M, Boreham, M, Stecher, J, Dullaers, M, Banchereau, J, Oh, S
    Mucosal Immunol, 2013;6(3):626-38.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  14. Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
    Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
    Int. J. Cancer, 2010;127(2):332-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009;69(15):6249-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
    Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
    Int. J. Cancer, 2008;122(4):769-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
    Authors: Bolovan-Fritts CA, Spector SA
    Blood, 2007;111(1):175-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
    Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
    J. Immunol., 2007;179(6):3724-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
    Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
    Cytokine, 2006;36(5):237-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11.
    Authors: Lepej SZ, Rode OD, Jeren T, Vince A, Remenar A, Barsic B
    J. Neuroimmunol., 2005;163(1):128-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2.
    Authors: Ottonello L, Montecucco F, Bertolotto M, Arduino N, Mancini M, Corcione A, Pistoia V, Dallegri F
    Cell. Signal., 2005;17(3):355-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004;114(11):1640-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003;73(5):584-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells.
    Authors: Howard OM, Dong HF, Yang D, Raben N, Nagaraju K, Rosen A, Casciola-Rosen L, Hartlein M, Kron M, Yiadom K, Dwivedi S, Plotz PH, Oppenheim JJ
    J. Exp. Med., 2002;196(6):781-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
    Authors: Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW
    J. Biol. Chem., 1999;274(14):9617-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Preferential recognition of monomeric CCR5 expressed in cultured cells by the HIV-1 envelope glycoprotein gp120 for the entry of R5 HIV-1.
    Authors: Nakano Y, Monde K, Terasawa H, Yuan Y, Yusa K, Harada S, Maeda Y
    Virology, 0;452(0):117-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human CCR5 Antibody

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Human CCR5 Antibody
By Anonymous on 02/07/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Brain endothelial cells Species: Human

Human CCR5 Antibody
By Anonymous on 07/01/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Melanoma tissue Species: Human

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