Human CCR5 Fluorescein-conjugated Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCR5.
  • Source
    Monoclonal Mouse IgG2B Clone # 45531
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CCR5
    Met1-Leu352
    Accession # P51681
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR5 in NS0 Mouse Cell Line Transfected with Human CCR5 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human CCR5 was stained with Mouse Anti-Human CCR5 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB182F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR5
CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for macrophage tropic HIV-1 infection.
  • Entrez Gene IDs:
    1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
  • Alternate Names:
    C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Involvement of the second extracellular loop and transmembrane residues of CCR5 in inhibitor binding and HIV-1 fusion: insights into the mechanism of allosteric inhibition.
    Authors: Maeda K, Das D, Yin PD, Tsuchiya K, Ogata-Aoki H, Nakata H, Norman RB, Hackney LA, Takaoka Y, Mitsuya H
    J. Mol. Biol., 2008;381(4):956-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. NF-kappaB modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gammadelta lymphocytes.
    Authors: Ferrarini M, Delfanti F, Gianolini M, Rizzi C, Alfano M, Lazzarin A, Biswas P
    J. Immunol., 2008;181(9):5857-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Inhibition of HIV-1 infection by viral chemokine U83A via high-affinity CCR5 interactions that block human chemokine-induced leukocyte chemotaxis and receptor internalization.
    Authors: Catusse J, Parry CM, Dewin DR, Gompels UA
    Blood, 2007;109(9):3633-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003;73(5):584-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Fluorescein‑conjugated Control Antibody

Ctrl IC0041F 6  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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