Human CD1a Alexa Fluor® 700-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human CD1a in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CD1d is observed and no cross-reactivity with with rhCD1b or rhCD1e is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 703217
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD1a
    Asp19-Val300 (predicted)
    Accession # P06126
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD1a in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CD1a Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB7076N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD1a
CD1a (also known as Leu6) ia a 48-50 kDa member of the five gene CD1 family of molecules. It is a type I transmembrane glycoprotein that is 311 amino acids (aa) in length. It contains three " alpha -domains" in its extracellular region (aa 17-300), and terminates with a short six aa segment. Although CD1a orthologs are found in multiple mammalian species, it does not appear to exist in rodent. Notably, not all humans possess a functional CD1a gene. CD1a serves as an MHC Class I type molecule that presents lipids to alpha beta TCRs on T cells. This means it exists as a heterodimer with beta 2-microglobulin on the cell surface. CD1a is unique in that it can present nonhydrophilic lipids to T cells; that is, there is no need for a polar "head" on one end of the lipid to interact with the polar residues of the TCR protein. Other cell membrane components suggested to associate with CD1a include CD74 and CD9, two molecules that may regulate cycling of CD1a. Cells that are known to express CD1a are restricted in number, and include cortical thymocytes, Langerhans cells, dermal dendritic cells (DC), and monocyte-derived DC. It has been suggested that type I mono-DCs, or those that secrete IL-12 and promote Th1 formation, are uniquely CD1a+. At this time, this appears to be too restrictive an assumption, and that the presence or absence of CD1a is only generally reflective of function.
  • Long Name:
    T Cell Surface Glycoprotein CD1a
  • Entrez Gene IDs:
    909 (Human)
  • Alternate Names:
    CD1; CD1a antigen; CD1A antigen, a polypeptide; CD1a molecule; CD1a; cluster of differentiation 1 A; cortical thymocyte antigen CD1A; differentiation antigen CD1-alpha-3; epidermal dendritic cell marker CD1a; FCB6; hTa1 thymocyte antigen; HTA1; R4; T6; T-cell surface antigen T6/Leu-6; T-cell surface glycoprotein CD1a
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 700-conjugated Antibody

Ctrl IC002N  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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