|Detection of CD21 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD21 PE‑conjugated Monoclonal Antibody (Catalog # FAB4909P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.|
CD21, also known as EBV Receptor or CR2 is a 145 kDa member of the RCA (Receptors of Complement Activation) family of proteins. It is expressed on T cells, B cells, and follicular dendritic cells. On the B cell surface, it combines with the BCR and CD19 to form a B cell activating complex. Mature human CD21 is 1013 amino acids (aa) in length. It is a type I Transmembrane (TM) protein that contains a 951 aa Extracellular Domain (ECD) (aa 21-971) and a short 34 aa cytoplasmic tail. The ECD exhibits fifteen 60 aa SUSHI repeats. Soluble CD21 can be generated by cleavage near the TM domain. One potential splice variant shows a deletion of aa 847‑908, while another shows an insertion of 59 aa after Lys659. The ECD of human CD21 shares 71% aa identity with mouse CD21 ECD.
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