Human CD300f/LMIR3 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human CD300f/LMIR3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LMIR1, 2, 4, 5, 6, or recombinant mouse LMIR3 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 234903
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Y3 rat myeloid cell line transfected with human CD300f/LMIR3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD300f/LMIR3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human CD300f/LMIR3 PE‑conjugated Monoclonal Antibody (Catalog # FAB2774P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD300f/LMIR3
CD300f, also known as CD300LF, LMIR3, IREM-1, CLM-1, IgSF13, DIgR1, and MAIR-V, is a 50‑60 kDa glycoprotein member of the immunoglobulin superfamily (1). Human CD300f consists of a 137 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 113 aa cytoplasmic domain that contains multiple immunoreceptor tyrosine-based inhibitory motifs (ITIMs) or ITIM-like sequences (2, 3). Alternate splicing generates additional isoforms that carry substituted C-terminal tails of varying lengths and sequences following the ECD or transmembrane segment (3). Within the ECD, human CD300f shares 43% aa sequence identity with mouse and rat CD300f. CD300f is expressed on the surface of dendritic cells, monocytes, granulocytes, and mast cells as well as on acute myeloid leukemia (AML) blasts (2-4). Pervanadate treatment or antibody crosslinking of CD300f induces phosphorylation of tyrosine residues in the cytoplasmic domain and the subsequent recruitment of phosphatases SHIP, SHP-1, SHP-2, and the p85 alpha subunit of PI3K (2, 3, 5, 6). CD300f ligation can induce cell death and inhibit signaling through multiple receptors including Fc epsilon RI, LMIR4, SCF R, TLR2, TLR3, and TLR9 (3-8). In contrast, it enhances TLR4-mediated signaling/cytokine production in mast cells through association with the activating signaling protein FcR gamma (5). In mouse, a splice variant of CD300f (known as DIgR2, with a 7 aa insertion in the ECD) inhibits CD4+ T cell activation and in vivo Th1 and CTL responses (9). CD300f is upregulated on monocytes surrounding experimentally-induced spinal cord demyelination and functions as a negative regulator of inflammation in the CNS (10).
  • References:
    1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
    2. Sui, L. et al. (2004) Biochem. Biophys. Res. Commun. 319:920.
    3. Alvarez-Errico, D. et al. (2004) Eur. J. Immunol. 34:3690.
    4. Korver, W. et al. (2009) Leukemia 23:1587.
    5. Izawa, K. et al. (2009) J. Immunol. 183:925.
    6. Alvarez-Errico, D. et al. (2007) J. Immunol. 178:808.
    7. Can, I. et al. (2008) J. Immunol. 180:207.
    8. Izawa, K. et al. (2007) J. Biol. Chem. 282:17997.
    9. Shi, L. et al. (2006) Blood 108:2678.
    10. Xi, H. et al. (2010) J. Exp. Med. 207:7.
  • Long Name:
    Leukocyte Mono Ig-like Receptor 3
  • Entrez Gene IDs:
    146722 (Human); 246746 (Mouse)
  • Alternate Names:
    CMRF35-like molecule 1; CD300 molecule-like family member f; CD300f; CD300LF; CLIM1; CLM1; CLM-1; DIgR2; IGSF13; IREM1; IREM-1; LMIR3; NKIR; Pigr3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human CD300f/LMIR3 PE-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information