Rat IgG2B PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC013P
Detection of Rat IgGControl by Flow Cytometry
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Rat IgG2B PE-conjugated Antibody Summary

Specificity
Selected for use as a rat IgG2B isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG2B Clone # 141945
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Rat IgGControl by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) were stainedwith Rat Anti-Human/Mouse CD44 PE-conjugated Monoclonal Antibody
(Catalog #FAB6127P,filled histogram) or Rat IgG PE-conjugated Isotype Control Antibody(Catalog # IC013P, open histogram). View our protocol for StainingMembrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

Product Datasheets

Citations for Rat IgG2B PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity
    Authors: Zaidoon Al-Jaderi
    Front Immunol, 2016;7(0):437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. AMPK-Activated Protein Kinase Suppresses Ccr2 Expression by Inhibiting the NF-kappaB Pathway in RAW264.7 Macrophages.
    Authors: Kumase F, Takeuchi K, Morizane Y, Suzuki J, Matsumoto H, Kataoka K, Al-Moujahed A, Maidana D, Miller J, Vavvas D
    PLoS ONE, 2016;11(1):e0147279.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Ctrl
  3. Chemokine receptor CX3CR1 contributes to macrophage survival in tumor metastasis.
    Authors: Zheng, Jiao, Yang, Min, Shao, Jianghua, Miao, Yanju, Han, Jiahuai, Du, Jie
    Mol Cancer, 2013;12(1):141.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow control

FAQs

No product specific FAQs exist for this product, however you may

View all Isotype Control FAQs

Reviews for Rat IgG2B PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Rat IgG2B PE-conjugated Antibody and earn rewards!

Have you used Rat IgG2B PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review