Human CD35 PE-conjugated Antibody

FAB5748P has been discontinued. View all CD35 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD35 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat CSMD1, recombinant human (rh) CSMD2, rhCSMD3, rhHABP1/C1QBP, or rhComplement Factor H is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 594708
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD35
    Gln42-Asp1971 (His1208Arg)
    Accession # CAA68755
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD35 in Human Whole Blood Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Mouse Anti-Human CD35 PE‑conjugated Monoclonal Antibody (Catalog # FAB5748P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD35

CD35, also known as Complement Receptor 1 (CR1), is a 220-300 kDa type I transmembrane glycoprotein member of the RCA (Regulators of Complement Activation) family of proteins. CD35 binds and internalizes particles and immune complexes that are opsonized with MBL or complement components C3b, C3i, C4b, or C1q. CD35 additionally protects the cell from complement-mediated lysis by serving as a cofactor for Factor I and inhibiting the C3 and C5 convertases. The extracellular domain (ECD) of human CD35 contains 30 tandem SCR/SUSHI repeats. A soluble form of the CD35 ECD circulates in the serum. There do not appear to be any splice variants associated with the CD35 gene. A mouse ortholog of human CD35 has not been described, although alternate splicing of mouse CD21/CR2 generates a protein with homology to some SCR repeats of human CD35. Cell surface CD35 is expressed on selective cells such as follicular dendritic cells, erythrocytes, astrocytes, kidney podocytes, neutrophils, and eosinophils.

  • Entrez Gene IDs:
    1378 (Human)
  • Alternate Names:
    C3BR; CD35; complement component (3b/4b) receptor 1 (Knops blood group); CR1; KN
Related Research Areas

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