Human CD19 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4867F
Human CD19 Fluorescein-conjugated Antibody in Data
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Product Details
Citations (3)
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Human CD19 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD19.
Source
Monoclonal Mouse IgG1 Clone # 4G7-2E3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human CLL cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD19 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD19 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F) or (B) Mouse IgG1Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD19

CD19 is a 95 kDa transmembrane glycoprotein with two Ig-like C2-set domains. CD19 regulates B cell development and activation through interactions with CD21, CD22, and the B cell receptor. CD19 polymorphisms and up‑regulation lead to the development of autoimmunity by promoting autoantibody production. Within the extracellular domain, human CD19 (Accession # P15391) shares 57% amino acid sequence identity with mouse and rat CD19.

Entrez Gene IDs
930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)
Alternate Names
B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12

Product Datasheets

Citations for Human CD19 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Assessing the expression of immunosuppressive cytokines in the newly diagnosed systemic lupus Erythematosus patients: a focus on B cells
    Authors: M Abbasifard, Z Kamiab, M Hasani, A Rahnama, P Saeed-Aska, H Khorramdel
    BMC Immunol, 2020;21(1):58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Effects of High Consumption of Vegetables on Clinical, Immunological, and Antioxidant Markers in Subjects at Risk of Cardiovascular Diseases
    Authors: I Peluso, A Raguzzini, G Catasta, V Cammisotto, A Perrone, C Tomino, E Toti, M Serafini
    Oxid Med Cell Longev, 2018;2018(0):5417165.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  3. Over-expression of receptor activator of nuclear factor-kappaB ligand (RANKL), inflammatory cytokines, and chemokines in periprosthetic osteolysis of loosened total hip arthroplasty.
    Authors: Wang CT, Lin YT, Chiang BL, Lee SS, Hou SM
    Biomaterials, 2010;31(1):77-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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