Human CD5 PE-conjugated Antibody

FAB1636P has been discontinued. View all CD5 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD5 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 205919
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD5
    Arg25-Asn371
    Accession # P06127
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD5 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD5 PE‑conjugated Monoclonal Antibody (Catalog # FAB1636P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD5

CD5, also known as Leu-1, is a type I transmembrane protein belonging to the Scavenger Receptor Cysteine-rich (SRCR) superfamily. It is expressed on thymocytes, T cells and the B1a subpopulation of B cells. CD5 binds the C-type lectin, CD72, and modulates signaling through the antigen receptors on T and B cells. CD5 has been shown to provide either positive or negative co-stimulatory signals depending on cell type to regulate immune responses.

  • References:
    1. Ledbetter, J.A. et al. (1985) J. Immunol. 135:2331.
    2. Damle, N.K. et al. (1988) J. Immunol. 140:1753.
  • Entrez Gene IDs:
    921 (Human); 12507 (Mouse)
  • Alternate Names:
    CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1
Related Research Areas

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