Human CD53 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4624R-100UG
Detection of CD53 in Human Peripheral Blood Lymphocytes by Flow Cytometry.
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Human CD53 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD53. Stains human CD53 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 425514
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CD53
Met1-Leu219
Accession # P19397
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD53 in Human Peripheral Blood Lymphocytes by Flow Cytometry. View Larger

Detection of CD53 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD53 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB4624R, filled histogram) or isotype control antibody (Catalog # IC003R, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD53

CD53, also known as TSPAN25, is a 35 kDa‑40 kDa cell surface and endosomal membrane glycoprotein in the tetraspanin superfamily. CD53 is widely expressed on hematopoietic cells. Ligation of CD53 promotes cell activation and survival as well as homotypic cell‑cell adhesion. CD53 associates with other tetraspanins, MHC class I and II molecules, and integrin alpha 4 beta 1. Human CD53 shares 81%‑83% aa sequence identity with mouse and rat CD53.

Entrez Gene IDs
963 (Human); 12508 (Mouse); 24251 (Rat)
Alternate Names
antigen MOX44 identified by monoclonal MRC-OX44; CD53 antigentetraspanin-25; CD53 glycoprotein; CD53 molecule; CD53 tetraspan antigen; CD53; cell surface antigen; Cell surface glycoprotein CD53; MOX44; MOX44transmembrane glycoprotein; Tetraspanin-25; TSPAN25; tspan-25; TSPAN25leukocyte surface antigen CD53

Product Datasheets

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