Human CD53 Alexa Fluor® 647-conjugated Antibody Summary
Accession # P19397
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD53 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD53 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB4624R, filled histogram) or isotype control antibody (Catalog # IC003R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CD53, also known as TSPAN25, is a 35 kDa‑40 kDa cell surface and endosomal membrane glycoprotein in the tetraspanin superfamily. CD53 is widely expressed on hematopoietic cells. Ligation of CD53 promotes cell activation and survival as well as homotypic cell‑cell adhesion. CD53 associates with other tetraspanins, MHC class I and II molecules, and integrin alpha 4 beta 1. Human CD53 shares 81%‑83% aa sequence identity with mouse and rat CD53.
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