Human CD53 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of CD53 in Human Peripheral Blood Lymphocytes by Flow Cytometry.
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Product Details
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Human CD53 APC-conjugated Antibody Summary

Species Reactivity
Detects human CD53. Stains human CD53 transfectants but not irrelevant transfectants.
Monoclonal Mouse IgG2A Clone # 425514
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human CD53
Accession # P19397
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD53 antibody in Human Peripheral Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD53 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD53 APC-conjugated Monoclonal Antibody (Catalog # FAB4624A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD53

CD53, also known as TSPAN25, is a 35 kDa‑40 kDa cell surface and endosomal membrane glycoprotein that belongs to the 4 TM tetraspanin superfamily of molecules. CD53 is widely expressed on hematopoietic cells, including eosinophils, neutrophils, mast cells, CD16+ proinflammatory monocytes, NK cells T cells, macrophages, splenic B cells and immature dendritic cells. Ligation of CD53 promotes cell proliferation and survival, as well as homotypic cell‑cell adhesion. On the cell membrane, CD53 associates with other tetraspanins, MHC class II molecules, and alpha 3 and alpha 4 integrins. Human CD53 shares 71% aa sequence identity with mouse and CD53 in the extracellular domains.

Entrez Gene IDs
963 (Human); 12508 (Mouse); 24251 (Rat)
Alternate Names
antigen MOX44 identified by monoclonal MRC-OX44; CD53 antigentetraspanin-25; CD53 glycoprotein; CD53 molecule; CD53 tetraspan antigen; CD53; cell surface antigen; Cell surface glycoprotein CD53; MOX44; MOX44transmembrane glycoprotein; Tetraspanin-25; TSPAN25; tspan-25; TSPAN25leukocyte surface antigen CD53

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