Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 425514
View all CD53 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CD53
Met1-Leu219
Accession # P19397

Specificity

Detects human CD53. Stains human CD53 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD53 APC‑conjugated Antibody

Detection of CD53 antibody in Human Peripheral Blood Lymphocytes antibody by Flow Cytometry.

Detection of CD53 in Human Peripheral Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD53 APC-conjugated Monoclonal Antibody (Catalog # FAB4624A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD53 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD53

CD53, also known as TSPAN25, is a 35 kDa‑40 kDa cell surface and endosomal membrane glycoprotein that belongs to the 4 TM tetraspanin superfamily of molecules. CD53 is widely expressed on hematopoietic cells, including eosinophils, neutrophils, mast cells, CD16+ proinflammatory monocytes, NK cells T cells, macrophages, splenic B cells and immature dendritic cells. Ligation of CD53 promotes cell proliferation and survival, as well as homotypic cell‑cell adhesion. On the cell membrane, CD53 associates with other tetraspanins, MHC class II molecules, and alpha 3 and alpha 4 integrins. Human CD53 shares 71% aa sequence identity with mouse and CD53 in the extracellular domains.

Alternate Names

CD53, MOX44, TSPAN25

Entrez Gene IDs

963 (Human); 12508 (Mouse); 24251 (Rat)

Gene Symbol

CD53

UniProt

P19397

Additional CD53 Products

Product Documents for Human CD53 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD53 APC‑conjugated Antibody

For research use only

Customer Reviews for Human CD53 APC‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Human CD53 APC‑conjugated Antibody and earn rewards!

Have you used Human CD53 APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies