Human CD9 Alexa Fluor™ Plus 647‑conjugated Antibody

R&D Systems | Catalog # FAB11464AFP647

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2665FF
View all CD9 Primary Antibodies »

Product Specifications

Specificity

Detects human CD9 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Human CD9 Alexa Fluor™ Plus 647‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CD9

CD9, also known as Tspan29, is a 24-27 kDa cell surface protein belonging to the tetraspanin family (1). Common to other tetraspanins, CD9 is composed of four transmembrane domains, short N- and C-terminal cytoplasmic domains, and two extracellular loops. The larger extracellular loop, referred to as the LEL or EC2, contains highly conserved CCG and PXSC motifs (2, 3). The LEL mediates noncovalent protein-protein interactions, allowing tetraspanins to associate with each other as well as signaling molecules, structural proteins, and G-protein coupled receptors (4-6). Human CD9 is expressed in multiple cell and tissue types and has been identified in diverse biological roles due to its involvement in the formation of tetraspanin-enriched microdomains (TEMs). TEMs are associated with numerous processes ranging from cell adhesion and fusion, membrane trafficking, and endocytosis to leukocyte adherence and motility (4-7). These tetraspanin-enriched microdomains (TEMs) are associated with a wide range of functions from cell adhesion and fusion, membrane trafficking and endocytosis, and eukocyte adherence and motility. The LEL of human CD9 shares 77% and 84% amino acid sequence identity with mouse and rat CD9, respectively.  CD9 can form homodimers or interact with other proteins including CD117, CD29, CD46, CD49c, CD81, CD315, Tspan4, TGF-alpha, and HBEGF (1, 4, 8-13). Increased expression of CD9 has been shown to enhance transmembrane TGF-alpha -induced EGFR stimulation (1), and injection of human CD9 mRNA into CD9 knock-out mouse oocytes restored sperm-egg fusion (14). CD9-LEL may also be involved in the inhibition of multinucleated giant cell formation (3) as well as possess anti-adhesive effects against bacteria trying to invade mammalian cells (6, 15). CD9 interacts with integrins to regulate cell adhesion and motility (16-18). CD9 has been implicated in platelet activation and aggregation (17, 19). It may act as the terminal signal of myelination in the peripheral nervous system and can regulate the formation of paranodal junctions (20). Also, it has been suggested CD9 plays an important role both in the self-antigen and recall antigen-induced T cell activation (21).

References

  1. Shi, W. et al. (2000) J. Cell Biol. 148:591.
  2. Hemler, M. (2003) Annu Rev Cell Biol. 19:397.
  3. Hulme, R. et al. (2014) PLoS One 9:e116289.
  4. Stipp, C. et al. (2003) Trends Biochem Sci. 28:106.
  5. Barreiro, O. et al. (2005) Blood 105:2852.
  6. Ventress, J. et al. (2016) PLoS One 11:e0160387.
  7. Rubinstein, E. (2011) Biochem Soc Trans. 39:501.
  8. Anzai, N. et al. (2002) Blood 99:4413.
  9. Radford, K. et al. (1996) Biochem. Biophys. Res. Commun. 222:13.
  10. Lozahic, S. et al. (2000) Eur. J. Immunol. 30:900.
  11. Park, K. et al. (2000) Mol. Hum. Reprod. 6:252.
  12. Charrin, S. et al. (2001) J. Biol. Chem. 276:14329.
  13. Tachibana, I. et al. (1997) J. Biol. Chem. 272:29181.
  14. Zhu, G. et al. (2002) Development 129:1995.
  15. Green, L. et al. (2011) Infect Immun. 79:2241.
  16. Powner, D. et al. (2011) Biochem. Soc. Trans. 39:563.
  17. Detchokul, S. et al. (2014) British Journal of Pharmacology 171:5462.
  18. Reyes, R. et al. (2018) Front. Immunol. 9:863.
  19. Slupsky, J. et al. (1989) J Biol chem. 264:12289.
  20. Ishibashi, T. et al. (2004) J. Neuroscience 24:96.
  21. Kobayashi, H. et al. (2004) Clin Exp Immunol. 137:101.

Alternate Names

CD9, DRAP-27, MIC3, p24, TSPAN29

Entrez Gene IDs

928 (Human); 12527 (Mouse)

Gene Symbol

CD9

UniProt

P21926

Additional CD9 Products

Product Documents for Human CD9 Alexa Fluor™ Plus 647‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD9 Alexa Fluor™ Plus 647‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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