Human CD94 Alexa Fluor® 350-conjugated Antibody

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FAB10581U-100UG
R&D Systems Antibodies
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Human CD94 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD94 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1032011
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell HEK293-derived human CD94
Lys32-Ile179
Accession # Q13241.2
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human Blood Lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitution default not known
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD94

CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation (1). Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain (2). Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions (3). Within the ECD, human CD94 shares 53% and 55% amino acid identity to mouse and rat CD94, respectively. CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8+ T cells (4). It associates into disulfide-linked heterodimers with NKG2A/B, C, or E (5-8), and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E (9, 10). Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively (11).

References
  1. Chester, C. et al. (2015) Front. Immunol. 6:601.
  2. Chang, C. et al. (1995) Eur. J. Immunol. 25:2433.
  3. Lieto, L.D. et al. (2006) Genes Immun. 7:36.
  4. Yu, J. et al. (2010) Blood 115:274.
  5. Phillips, J.H. et al. (1996) Immunity 5:163.
  6. Lazetic, S. et al. (1996) J. Immunol. 157:4741.
  7. Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
  8. Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
  9. Braud, V.M. et al. (1998) Nature 391:795.
  10. Kraemer, T. et al. (2014) J. Immunol. Res. 2014:352160.
  11. Houchins, J.P. et al. (1997) J. Immunol. 158:3603.
Entrez Gene IDs
3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)
Alternate Names
CD94 antigen; CD94; CD94natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KLRD1; KP43; NK cell receptor

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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