Human CD94 Antibody

R&D Systems | Catalog # MAB10581

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1032011
View all CD94 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived human CD94
Lys32-Ile179
Accession # Q13241.2

Specificity

Detects human CD94 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD94 Antibody

Detection of CD94 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD94 Monoclonal Antibody (Catalog # MAB10581) or (B) Mouse IgG2B isotype control antibody (MAB0041) followed by anti-Mouse IgG Allophycocyanin-conjugated secondary antibody (F0101B) and Mouse Anti-Human CD56 Phycoerythrin-conjugated Monoclonal Antibody (FAB24086P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human CD94 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human Blood Lymphocytes

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD94

CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation (1). Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain (2). Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions (3). Within the ECD, human CD94 shares 53% and 55% amino acid identity to mouse and rat CD94, respectively. CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8+ T cells (4). It associates into disulfide-linked heterodimers with NKG2A/B, C, or E (5-8), and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E (9, 10). Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively (11).

References

  1. Chester, C. et al. (2015) Front. Immunol. 6:601.
  2. Chang, C. et al. (1995) Eur. J. Immunol. 25:2433.
  3. Lieto, L.D. et al. (2006) Genes Immun. 7:36.
  4. Yu, J. et al. (2010) Blood 115:274.
  5. Phillips, J.H. et al. (1996) Immunity 5:163.
  6. Lazetic, S. et al. (1996) J. Immunol. 157:4741.
  7. Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
  8. Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
  9. Braud, V.M. et al. (1998) Nature 391:795.
  10. Kraemer, T. et al. (2014) J. Immunol. Res. 2014:352160.
  11. Houchins, J.P. et al. (1997) J. Immunol. 158:3603.

Alternate Names

CD94, KLRD1

Entrez Gene IDs

3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)

Gene Symbol

KLRD1

UniProt

Q13241.2

Additional CD94 Products

Product Documents for Human CD94 Antibody

Certificate of Analysis

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Product Specific Notices for Human CD94 Antibody

For research use only

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