Human CDCP1 APC-conjugated Antibody

Name: Human CDCP1 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB26663A

Catalog #: FAB26663A Datasheet / COA / SDS

Newer Version Available: FAB26663R

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Discontinued Product

FAB26663A has been discontinued and is replaced by FAB26663R.

Detection of CDCP1 in SW480 Human Cell Line by Flow Cytometry.

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Human CDCP1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CDCP1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CDCP1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 309116

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CDCP1
Ala33-Leu666
Accession # NP_073753

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CDCP1 in SW480 Human Cell Line by Flow Cytometry. SW480 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human CDCP1 APC-conjugated Monoclonal Antibody (Catalog # FAB26663A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CDCP1

CDCP1, also known as SIMA135 and CD318, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 amino acid (aa) extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted form of CDCP1 is also generated. An amino-terminal region of approximately 265 aa can also be proteolytically cleaved and released as a 65 kda fragment. Notably, the retained 70 kDa membrane-bound fragment serves as a signal-transducing receptor for Integrin alpha 2 beta 1. CDCP1 is found on the surface of colonic epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa sequence identity with that of the mouse protein.

Long Name

CUB Domain Containing Protein 1

Entrez Gene IDs

64866 (Human); 109332 (Mouse)

Alternate Names

CD318 antigen; CD318; CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; SIMA135TRASK; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases

Product Datasheets

Product Datasheet

COA

SDS

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