Human CDCP1 PE-conjugated Antibody

FAB26663P has been discontinued. View all CDCP1 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CDCP1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CDCP1 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 309116
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CDCP1
    Ala33-Leu666
    Accession # NP_073753
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CDCP1 in SW480 Human Cell Line by Flow Cytometry. SW480 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human CDCP1 PE‑conjugated Monoclonal Antibody (Catalog # FAB26663P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CDCP1
CDCP1, also known as SIMA135 and CD318, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 amino acid (aa) extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted form of CDCP1 is also generated. An amino-terminal region of approximately 265 aa can also be proteolytically cleaved and released as a 65 kda fragment. Notably, the retained 70 kDa membrane-bound fragment serves as a signal-transducing receptor for Integrin alpha 2 beta 1.  CDCP1 is found on the surface of colonic epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa sequence identity with that of the mouse protein.
  • Long Name:
    CUB Domain Containing Protein 1
  • Entrez Gene IDs:
    64866 (Human); 109332 (Mouse)
  • Alternate Names:
    CD318 antigen; CD318; CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; SIMA135TRASK; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases
Related Research Areas

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