Human CDCP1 PE-conjugated Antibody

Discontinued Product

FAB26663P has been discontinued.
Detection of CDCP1 in SW480 Human Cell Line by Flow Cytometry.
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Human CDCP1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CDCP1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CDCP1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 309116
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CDCP1
Ala33-Leu666
Accession # NP_073753
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CDCP1 in SW480 Human Cell Line by Flow Cytometry. View Larger

Detection of CDCP1 in SW480 Human Cell Line by Flow Cytometry. SW480 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human CDCP1 PE‑conjugated Monoclonal Antibody (Catalog # FAB26663P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CDCP1

CDCP1, also known as SIMA135 and CD318, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 amino acid (aa) extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted form of CDCP1 is also generated. An amino-terminal region of approximately 265 aa can also be proteolytically cleaved and released as a 65 kda fragment. Notably, the retained 70 kDa membrane-bound fragment serves as a signal-transducing receptor for Integrin alpha 2 beta 1.  CDCP1 is found on the surface of colonic epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa sequence identity with that of the mouse protein.

Long Name
CUB Domain Containing Protein 1
Entrez Gene IDs
64866 (Human); 109332 (Mouse)
Alternate Names
CD318 antigen; CD318; CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; SIMA135TRASK; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases

Product Datasheets

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