Human Claudin-10b Antibody (394513)

Name: Human Claudin-10b Antibody (394513)
Brand: R&D Systems
SKU: MAB4279

R&D Systems | Catalog # MAB4279

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Product Documents

Discontinued Product

MAB4279 has been discontinued. View all Claudin-10b products.

Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 394513

View all Claudin-10b Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Claudin‑10 b Isoform
Met1-Val228
Accession # P78369

Specificity

Detects human Claudin‑10b. Stains human Claudin‑10b transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Claudin-10b Antibody (394513)

Detection of Claudin‑10 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Claudin-10 b Isoform Monoclonal Antibody (Catalog # MAB4279, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Applications for Human Claudin-10b Antibody (394513)

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: HepG2 human hepatocellular carcinoma cell line

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Claudin-10b

Claudin-10 is a 228 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Two splice forms of human Claudin-10 have been described with a divergent N-terminal extracellular loop. Claudin-10b (Swiss-Prot isoform 1) is relatively cation-permeant compared to Claudin-10a and is widely expressed, while Claudin‑10a is restricted to the kidney cortex. Claudin-10 is overexpressed in some carcinomas. Human Claudin‑10b shares 94% aa sequence identity with mouse and rat Claudin-10.