Human Claudin-10b Antibody Summary
Accession # P78369
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Claudin‑10 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Claudin‑10 b Isoform Monoclonal Antibody (Catalog # MAB4279, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Claudin-10 is a 228 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Two splice forms of human Claudin-10 have been described with a divergent N-terminal extracellular loop. Claudin-10b (Swiss-Prot isoform 1) is relatively cation-permeant compared to Claudin-10a and is widely expressed, while Claudin‑10a is restricted to the kidney cortex. Claudin-10 is overexpressed in some carcinomas. Human Claudin‑10b shares 94% aa sequence identity with mouse and rat Claudin-10.
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