Human Claudin-10b Antibody

Catalog # Availability Size / Price Qty
MAB4279
MAB4279-SP
Detection of Claudin‑10 in HepG2 Human Cell Line by Flow Cytometry.
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Human Claudin-10b Antibody Summary

Species Reactivity
Human
Specificity
Detects human Claudin‑10b. Stains human Claudin‑10b transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 394513
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Claudin‑10 b Isoform
Met1-Val228
Accession # P78369
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Claudin‑10 in HepG2 Human Cell Line by Flow Cytometry. View Larger

Detection of Claudin‑10 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Claudin‑10 b Isoform Monoclonal Antibody (Catalog # MAB4279, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-10b

Claudin-10 is a 228 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Two splice forms of human Claudin-10 have been described with a divergent N-terminal extracellular loop. Claudin-10b (Swiss-Prot isoform 1) is relatively cation-permeant compared to Claudin-10a and is widely expressed, while Claudin‑10a is restricted to the kidney cortex. Claudin-10 is overexpressed in some carcinomas. Human Claudin‑10b shares 94% aa sequence identity with mouse and rat Claudin-10.

Entrez Gene IDs
9071 (Human); 58187 (Mouse); 290485 (Rat)
Alternate Names
Claudin10b; Claudin-10b; CLDN10; CPETRL3; OSP-L

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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