Human CLEC4D/CLECSF8 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF2806R Datasheet / COA / SDS

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AF2806R-100UG

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Human CLEC4D/CLECSF8 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CLEC4D/CLECSF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) CLECSF9 and rhCLEC4F is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CLEC4D/CLECSF8
Gly52-Asn215
Accession # Q8WXI8

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CLEC4D/CLECSF8

Human CLEC4D, also known as MCL and CLECSF8, is a 30 kDa type II transmembrane (TM) glycoprotein that belongs to the CLR (C-type Lectin Receptor) family of molecules. It is synthesized as a 215 amino acid (aa) protein that contains a 17 aa N-terminal cytoplasmic domain, a 21 aa TM segment, and a 177 aa C-terminal extracellular region. The extracellular region shows a short stalk and a 118 aa CRD (carbohydrate recognition domain). The nature of its carbohydrate ligand is unknown. CLEC4F is restricted to monocytes/macrophages and serves as an endocytic receptor. Homodimers and homotrimers form on the cell surface. The human CLEC4D extracellular region shares 63% aa sequence identity with the mouse extracellular region.

Long Name

C-type Lectin Domain Family 4, Member D

Entrez Gene IDs

338339 (Human); 17474 (Mouse)

Alternate Names

CD368; CLEC4D; CLEC6; CLEC-6; CLECSF8; CLECSF8Mpcl; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 8; C-type lectin domain family 4 member D; C-type lectin domain family 4, member D; C-type lectin receptor; C-type lectin superfamily member 8; C-type lectin-like receptor 6; macrophage C-type lectin; MCL; MGC40078; Mpcl